土壤源孔雀石绿脱色菌的筛选及脱色条件分析  

Screening of soil-derived bacteria decolorizing malachite green and decoloring condition analysis

作　　者：廖华媛[1] 熊梦霞 李润成[1] LIAO Hua-yuan;XIONG Meng-xia;LI Run-cheng(College of Veterinary Medicine,Hunan Agricultural University,Changsha410128,China)

机构地区：[1]湖南农业大学动物医学院,长沙410128

出　　处：《湖北农业科学》2020年第22期86-90,96,共6页Hubei Agricultural Sciences

基　　金：国家重点研发项目(2017YFD0500102)。

摘　　要：从土壤来源的细菌中,筛选可使孔雀石绿(MG)脱色的菌株,结果得到1株阴沟肠杆菌(Enterobacter cloacae)(S1-2)对MG有较强的脱色能力。通过MG被脱色前后溶液吸光度的变化衡量脱色率,用单一变量法分析影响菌株S1-2发挥MG脱色作用的不同条件。结果表明,菌株S1-2在36 h内可使1100 mg/L的MG完全脱色,处理后的MG对解淀粉芽孢杆菌、壁芽孢杆菌抑制率降低了43.98%~48.21%。用300 mg/L的MG进行脱色条件分析发现,按0.5%~7.0%接菌,脱色率达91.67%~93.91%;不同pH培养环境中,该菌在pH 6、7时对MG脱色率为90.02%~94.54%;在20~40℃条件下培养,该菌对MG脱色率达84.61%~94.56%;在6~18 g/L的NaCl浓度下,该菌对MG的脱色率达91.85%~93.77%。To screen bacteria capable of decolorizing malachite green(MG)from the soil-derived bacteria,as a result,a strain of Enterobacter cloacae(S1-2)had a strong ability to decolorize MG.According to single variable principle,the different conditions that affected the strain S1-2 were characterized by the change in absorbance of the solution before and after MG degradation.The results showed that the strain S1-2 could completely decolorize 1100 mg/L MG within 36 h,and the inhibitory rate of treated MG on Bacillus amyloliquefaciens and Bacillus parvii decreased by 43.98%~48.21%.The analysis of decolorization conditions with 300 mg/L MG found that the degradation rate of 0.5%~7.0%inoculation amount of strain S1-2 was 91.67%~93.91%;In different pH culture environment,the strain S1-2 decolorized 90.02%~94.54%MG at pH 6 and pH 7;The strain S1-2 decomposed 84.61%~94.56%MG,when cultured at 20~40℃;Under the concentration of 6~18 g/L NaCl,the strain S1-2 degraded 91.85%~93.77%MG.

关 键 词：孔雀石绿 阴沟肠杆菌(Enterobacter cloacae) 毒性测试 脱色条件 

分 类 号：X172[环境科学与工程—环境科学] X788