α-硫辛酸对实验性自身免疫性脑脊髓炎大鼠脊髓内NF-κB表达的影响  

Effects of alpha lipoic acid on the expression of NF-κB in the spinal cord of rats with experimental autoimmune encephalomyelitis

作　　者：檀红玲[1] 梁丹 檀国军[2] 马玉腾[1] 韩梅[1] 高梦颖[1] 张军立[1] Tan Hongling;Liang Dan;Tan Guojun;Ma Yuteng;Han Mei;Gao Mengying;Zhang Junli(Second Hospital of Hebei Medical University,Shijiazhuang,Hebei Province,Shijiazhuang 050000,China;不详)

机构地区：[1]河北医科大学第二医院急诊重症医学科,石家庄050000 [2]河北医科大学第二医院神经内科,石家庄050000

出　　处：《疑难病杂志》2020年第12期1261-1265,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省卫生健康委科研基金项目(20190558)。

摘　　要：目的探究α-硫辛酸(α-LA)对实验性自身免疫性脑脊髓炎(EAE)大鼠脊髓内核因子κB(NF-κB)表达的影响。方法2019年1—12月于河北医科大学第二医院神经病学实验室进行实验。选择成年健康雌性Wistar大鼠42只,随机数字表法分为空白对照组(NC组)6只,不予任何处理。余大鼠36只用新鲜豚鼠全脊髓匀浆(GPSCH)诱导免疫,建立多发性硬化动物模型。待大鼠发病后随机数字表法分为EAE组、α-硫辛酸组(α-LA组),每组18只;α-硫辛酸组每日腹腔注射α-硫辛酸,NC组、EAE组每日腹腔注射等体积的生理盐水,均干预7 d。比较各组大鼠神经功能情况,观察脊髓腰膨大组织病理形态变化,脊髓腰膨大组织中NF-κB及其抑制蛋白IκB的表达,大鼠血清炎性细胞因子水平。结果与EAE组比较,α-LA组大鼠发病后0、7及14 d时神经功能评分均降低(F=47.763、37.101、51.620,P均<0.001)。与NC组比较,EAE组NF-κB表达明显升高,IκB表达明显降低;与EAE组比较,α-LA组各时期NF-κB表达均降低,IκB表达均升高(F/P=86.431/<0.001,32.921/0.003)。与NC组比较,EAE组血清IL-2、IL-1β和TNF-α水平均升高;与EAE组比较,α-LA组各时点血清炎性因子水平均降低(F=26.288、211.859、38.378,P均<0.01)。结论α-硫辛酸可明显减轻EAE大鼠神经损伤,降低炎性反应水平,其机制可能与下调NF-κB表达,并上调IκB表达有关。Objective To explore the effect of alpha lipoic acid(α-LA)on the expression of nuclear factorκB(NF-κB)in the spinal cord of rats with experimental autoimmune encephalomyelitis(EAE).Methods Experiments were conducted in the Neurology Laboratory of the Second Hospital of Hebei Medical University from January to December 2019.Forty-two adult healthy female Wistar rats were selected and divided into 6 blank control group(NC group)by random number table method without any treatment.The remaining 36 rats were immunized with fresh guinea pig whole spinal cord homogenate(GPSCH)to establish multiple sclerosis animal models.The rats were randomly divided into EAE group andα-lipoic acid group(α-LA group)after the onset of the disease,with 18 rats in each group.Theα-lipoic acid group was intraperitoneally injected withα-lipoic acid daily,and the NC group and EAE group were intraperitoneally injected with an equal volume of normal saline daily for 7 days of intervention.The neurological function of rats in each group was compared,the pathological changes of spinal lumbar enlargement tissue were observed,the expression of NF-κB and its inhibitory protein IκB in spinal lumbar enlargement tissue,and the levels of serum inflammatory cytokines in rats.Results Compared with the EAE group,the neurological scores of rats in theα-LA group decreased at 0,7,and 14 days after the onset of the disease(F=47.763,37.101,51.620,all P<0.001).Compared with the NC group,the expression of NF-κB in the EAE group was significantly increased,and the expression of IκB was significantly decreased.Compared with the EAE group,the expression of NF-κB in theα-LA group decreased in each period,and the expression of IκB increased(F/P=86.431/<0.001,32.921/0.003).Compared with the NC group,serum IL-2,IL-1βand TNF-αlevels in the EAE group were increased.Compared with the EAE group,the serum inflammatory factor levels in theα-LA group decreased at each time point(F=26.288,211.859,38.378,all P<0.01).Conclusion Alpha lipoic acid can significantl

关 键 词：Α-硫辛酸 实验性自身免疫性脑脊髓炎 核因子-ΚB IΚB 大鼠 

分 类 号：R74[医药卫生—神经病学与精神病学]