多肽荧光传感器检测铜离子  

Fluorescent Peptide Sensor for Detection of Copper Ions

作　　者：阿丽 王勇 A Li;WANG Yong(College of Chemistry,Nanchang University,Nanchang 330031,China;Jiangxi Province Key Laboratory of Modern Analytical Science,Nanchang University,Nanchang 330031,China)

机构地区：[1]南昌大学化学学院,南昌330031 [2]南昌大学现代分析科学江西省重点实验室,南昌330031

出　　处：《高等学校化学学报》2020年第12期2736-2741,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21864017,31960495);江西省科技创新平台项目(批准号:20192BCD40001);南昌大学食品科学与技术国家重点实验室基金(批准号:SKLF-KF-201810);湖南大学生物传感及化学计量学重点实验室基金(批准号:SKLCBC-2018007)资助.

摘　　要：基于铜离子对罗丹明B标记的多肽的荧光猝灭作用,构建了一种用于铜离子检测的荧光传感器.利用共振光散射分析、紫外-可见吸收光谱、荧光寿命测试和圆二色光谱研究了铜离子检测的传感机理,发现铜离子的加入诱导多肽结构发生变化而使罗丹明B荧光团彼此靠近,从而导致铜离子与多肽之间发生聚集诱导荧光猝灭.实验结果表明,该传感器检测铜离子的线性范围为5×10‒4~1×10‒2μmol/L和0.1~7μmol/L,检出限为0.29 nmol/L,且拥有良好的选择性,能用于湖水样品中铜离子的检测.A fluorescent sensor for copper(Ⅱ)ions was developed based on the quenching of rhodamine B-labeled peptide by copper(Ⅱ).The sensing mechanism was studied using resonance light scattering,UV-Vis absorption spectroscopy,fluorescence lifetime,and circular dichroism.The results showed that copper(Ⅱ)might induced the conformational change of the peptide that make rhodamine B approach each other,resulting in the aggregation-caused quenching effect of copper(Ⅱ)and the rhodamine B-labeled peptide.More⁃over,the experimental results displayed that the sensor possessed both linear ranges of 5×10‒4—1×10‒2μmol/L and 0.1—7μmol/L with a detection limit of 0.29 nmol/L.The sensor also had a high selectivity for copper(Ⅱ),and can be successfully employed for the detection of copper(Ⅱ)ions in lake water samples.

关 键 词：荧光传感器 多肽 铜离子 

分 类 号：O657[理学—分析化学]