苏木乙酸乙酯提取物对动脉粥样硬化模型大鼠总胆固醇及白细胞介素-18的影响  

Influences of Ethyl Acetate Extract of Hematoxylin on TC and IL-18 in Atherosclerosis Rats

作　　者：袁野[1] 范增光 YUAN Ye;FAN Zengguang(Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中西医结合心脑血管病杂志》2020年第22期3780-3782,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金(No.81102590);黑龙江省教育厅人才项目(No.UNPYSCT-2018228)。

摘　　要：目的观察苏木乙酸乙酯提取物对动脉粥样硬化(AS)模型大鼠总胆固醇(TC)及外周血白细胞介素-18(IL-18)水平的影响,探讨其抗AS的部分机制。方法将40只大鼠随机分为空白组、模型组、苏木乙酸乙酯提取物组及辛伐他汀组,每组10只,空白组、模型组均灌服等体积的羧甲基纤维素钠10 mL/(kg·d);辛伐他汀组灌服含0.42 mg/mL辛伐他汀的羧甲基纤维素钠混悬剂10 mL/(kg·d);苏木乙酸乙酯提取物组灌服含生药0.115 mg/mL的羧甲基纤维素钠10 mL/(kg·d)。连续灌胃4周后抽取各组大鼠腹主动脉血液,得到血清,测定TC水平;应用酶联免疫吸附实验(ELISA)检测血清IL-18水平。结果与模型组对比,苏木乙酸乙酯提取物组可以明显降低TC及血清IL-18水平(P<0.05);苏木乙酸乙酯提取物组TC、IL-18水平与辛伐他汀组比较差异无统计学意义(P>0.05)。结论苏木乙酸乙酯提取物可以降低AS模型大鼠TC及IL-18水平,具有较好的降低血脂及免疫抑制作用。Objective To observe the effects of ethyl acetate extract of hematoxylin on the levels of total cholesterol(TC)and interleukin-18(IL-18)in peripheral blood of atherosclerosis(AS)model rats,and to explore the mechanisms of its anti-AS.Methods The rats were randomly divided into blank group,model group,ethyl acetate extract of hematoxylin group,and simvastatin group,with 10 rats in each group.Both the rats in blank group and model group were given an equal volume of sodium carboxymethyl cellulose 10 mL/(kg·d).The rats in simvastatin group were given infusion of 0.42 mg/mL simvastatin sodium carboxymethyl cellulose suspension 10 mL/(kg·d).The rats in ethyl acetate extract of hematoxylin group were given administer 10 mL/(kg·d)of sodium carboxymethyl cellulose suspension containing crude drug 0.115 g/mL.After 4 weeks of continuous intragastric administration,the abdominal aorta blood of rats was extracted and serum was obtained,TC level was determined,and IL-18 level was detected by enzyme linked immunosorbent assay.Results Compared with model group,ethyl acetate extract of hematoxylin could significantly reduce the level of TC and serum IL-18(P<0.05),and there was no significant difference in TC and serum IL-18 levels between ethyl acetate extract of hematoxylin group and simvastatin group(P>0.05).Conclusion Ethyl acetate extract of hematoxylin can reduce the levels of TC and IL-18 in AS model rats,and has a better effect on reducing blood lipid and immunosuppressive effect.

关 键 词：动脉粥样硬化 苏木乙酸乙酯提取物 辛伐他汀 总胆固醇 白细胞介素-18 实验研究 

分 类 号：R743[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]