长春花接种丛枝菌根真菌对柑橘黄龙病菌的抑制效果  

Effects of Inhibition Citrus Huanglongbing on Catharanthus roseus with Different Arbuscular Mycorrhizal Fungi Species

作　　者：全达万 李栋 张金莲 宋娟[4] 胡柳[2] 程通 黄京华[1] 陈廷速[4] QUAN Dawan;LI Dong;ZHANG Jinlian;SONG Juan;HU Liu;CHENG Tong;HUANG Jinghua;CHEN Tingsu(School of Agronomy,Guangxi University,Nanning,Guangxi 530001,China;Zhejiang Yangshengtang Institute of Natural Medication Coopration,Hangzhou,Zhejiang 310007,China;School of Public Health Care,Xiamen University,Xiamen,Fujian 361102,China;Microbiology Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西大学农学院,广西南宁530001 [2]浙江养生堂天然药物研究所有限公司,浙江杭州310007 [3]厦门大学公共卫生学院,福建厦门361102 [4]广西农业科学院微生物研究所,广西南宁530007

出　　处：《热带作物学报》2020年第11期2259-2266,共8页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31360356,No.31760137);厦门大学联合项目(2017-21);广西农业科学院科技项目(No.2015YT81)。

摘　　要：通过筛选获得具有显著促生和抗病能力的AM真菌菌株,为防治柑橘黄龙病提供理论依据和基础,并探索防治新途径。采用盆栽法,于长春花幼苗期分别接种17株AM真菌进行长春花农艺性状和对柑橘黄龙病的抗病性比较试验,研究不同丛枝菌根真菌菌株对长春花的促生效果及对柑橘黄龙病的抑制效果。结果表明,长春花接种AM真菌后,菌株GZ10、GZ5和JX70能显著促进植株生长,其中接种GZ10的株高和茎径分别比CK增加了23.1%和12.1%,接种GZ5的叶片数比CK增加了12.9%;接种菌株GZ1、BNMJ和PWJ有效提高了长春花对柑橘黄龙病的抗病和耐病能力,其相对防效分别为78.39%、66.87%和69.75%。To provide fundamental references and better ways of preventing and controlling citrus Huanglongbing(HLB),a research was conducted to obtain arbuscular mycorrhizal(AM)fungi that possess plant growth-promoting and disease resistance abilities.The experiment utilized the pot culture method,where 17 strains of AM fungi were inoculated into the roots of Catharanthus roseus that were in the seedling stage.The colonization of different AM fungi strains were analyzed to understand how AM fungi simulating the growth and ameliorate citrus HLB infection in C.roseus under greenhouse conditions.During this process,various measurements were taken,and the agronomic characters,rate of colonization,infection intensity,arbuscular abundance,and HLB infection were analyzed.After C.roseus plants were inoculated with AM strains,strains GZ10,GZ5,and JX70 significantly enhanced the growth of C.roseus.Plants inoculated with GZ10 exhibited 23.1%and 12.1%increase in stem height and diameter,while GZ5 increased leaf number by 12.9%,both in respect to CK.Three strains(GZ1,BNMJ,and PWJ)improved disease resistance to citrus HLB,with a relative prevention rate of 78.39%for GZ1,66.87%for BNMJ,and 69.75%for PWJ.The result proved that AM inoculation could significantly improve the growth of C.roseus and to some extent increase its disease resistance to citrus HLB.

关 键 词：长春花 丛枝菌根真菌 优势菌株 柑桔黄龙病 抗性 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]