大蒜素预处理对急性心肌梗死模型大鼠心肌病理改变的影响  

Effect of Allicin Pretreatment on Myocardial Pathological Changes in Rats with Acute Myocardial Infarction

作　　者：方燕 郑旭旭 李丽燕[1] FANG Yan;ZHENG Xuxu;LI Liyan(Department of Pathology,Wenzhou Hospital of Traditional Chinese Medicine,Wenzhou,Zhejiang province,325000,China)

机构地区：[1]浙江省温州市中医院病理科,温州325000

出　　处：《浙江中西医结合杂志》2020年第12期957-962,I0009,I0010,共8页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省温州市科技局科研基金资助项目(No.2019Y0874)。

摘　　要：目的研究大蒜素预处理对急性心肌梗死(AMI)模型大鼠心肌组织病理改变的影响。方法180只SD大鼠按随机数字表法分为假手术组(生理盐水预处理)、AMI组(生理盐水预处理)、维拉帕米2.5mg·kg^-1·d^-1预处理组和大蒜素5、10、20mg·kg^-1·d^-1预处理组,每组30只;采用结扎左冠状动脉前降支的方法复制AMI大鼠模型,造模手术前7天开始腹腔注射给药预处置。造模24h后,通过超声影像检测各组大鼠左心室舒张末期内径(LVIDd)和收缩末期左室内径(LVIDs)、射血分数(EF)和每搏输出量(SV),计算心脏指数,硝基蓝四氮唑(NBT)染色法计算心肌梗死体积百分比,苏木精-伊红(HE)染色法行心肌组织病理学检查并进行损伤评分,原位末端转移酶标记(TUNEL)法观察细胞凋亡状况并计算凋亡指数(AI),透射电子显微镜观察心肌细胞超微结构变化。结果与AMI组比较,经大蒜素10、20mg·kg^-1·d^-1或维拉帕米2.5mg·kg^-1·d^-1预处理7天能够明显抑制AMI大鼠LVIDd[(6.93±0.46)mm、(6.84±0.42)mm、(6.87±0.45)mm比(7.42±0.50)mm,P均<0.05]、LVIDs[(5.47±0.48)mm、(4.62±0.41)mm、(5.13±0.45)mm比(6.09±0.57)mm,P<0.05或P<0.01]升高和EF[(39.76±8.14)%、(47.08±8.65)%、(43.91±8.40)%比(31.12±5.64)%,P<0.05或P<0.01]、SV[(102.63±21.58)μL、(129.19±23.17)μL、(107.05±22.83)μL比(83.74±18.59)μL,P<0.05或P<0.01]降低,抑制心脏指数[(2.63±0.28)×10-3、(2.44±0.25)×10^-3、(2.64±0.27)×10^-3比(2.91±0.30)×10^-3,P<0.05或P<0.01]和心肌梗死体积百分比[(12.54±2.03)%、(5.18±1.02)%、(11.62±1.84)%比(22.75±3.16)%,P均<0.01]升高,抑制心肌组织缺血性病变及损伤评分[(5.94±1.01)分、(3.76±0.85)分、(4.82±0.93)分比(7.24±1.18)分,P<0.05或P<0.01]升高,抑制心肌细胞AI[(23.50±3.79)%、(13.07±1.82)%、(21.63±2.47)%比(43.83±4.75)%,P均<0.01]升高,抑制心肌细胞超微结构病变。与维拉帕米2.5mg·kg^-1·d^-1预处理组比较,大蒜素20mg·kg^-1·d^-1预处理对AMI模型大�Objective To explore the effect of allicin pretreatment on myocardial pathological changes in rats with acute myocardial infarction(AMI).Methods One hundred and eighty SD rats were randomly divided into sham operation group(saline pretreatment),AMI group(saline pretreatment),2.5mg/(kg·d)verapamil pretreatment group,and 5,10 and 20mg/(kg·d)allicin pretreatment group,with 30 animals in each group.AMI rat model was established by ligating the anterior descending branch of the left coronary artery,and intraperitoneal injection was given 7 days before the operation.Twenty-four hours after modeling,the left ventricular end-diastolic diameter(LVIDd)and end-systolic left ventricular diameter(LVIDs),ejection fraction(EF)and stroke volume(SV)of each group of rats were detected by ultrasound imaging,and the heart index was calculated.Histopathological examination and injury score were performed by hematoxylin eosin(HE)staining.Apoptosis was investigated by in situ terminal transferase labeling(TUNEL)and apoptosis index(AI)was calculated.Ultrastructural changes of myocardial cells were observed by transmission electron microscope.Results Compared to AMI group,pretreatment with 10 and 20mg/(kg·d)allicin or 2.5mg/(kg·d)verapamil for 7d significantly inhibited the increase of LVIDd[(6.93±0.46),(6.84±0.42)and(6.87±0.45)vs(7.42±0.50)mm,P<0.05,respectively],LVIDs[(5.47±0.48),(4.62±0.41)and(5.13±0.45)vs(6.09±0.57)mm;P<0.05 or P<0.01];the decrease of EF[(39.76±8.14)%,(47.08±8.65)%and(43.91±8.40)%vs(31.12±5.64)%,P<0.05 or P<0.01],SV[(102.63±21.58),(129.19±23.17)and(107.05±22.83)vs(83.74±18.59)μL,P<0.05 or P<0.01];the increase of cardiac index[(2.63±0.28)×10-3,(2.44±0.25)×10^-3 and(2.64±0.27)×10^-3 vs(2.91±0.30)×10^-3,P<0.05 or P<0.01]and myocardial infarction volume percentage[(12.54±2.03)%,(5.18±1.02)% and(11.62±1.84)%vs(22.75±3.16)%,P<0.01,respectively];the increase of myocardial ischemic lesions and injury scores[(5.94±1.01),(3.76±0.85)and(4.82±0.93)vs(7.24±1.18),P<0.05 or P<0.01];the increase

关 键 词：大鼠 大蒜素 急性心肌梗死 病理 细胞凋亡 超微结构 

分 类 号：R285.5[医药卫生—中药学]