橙皮苷对口腔鳞状细胞癌裸鼠移植瘤的抑制作用及其与紫杉醇联合应用的作用  

The inhibitory effects of hesperidin and its combination effects with paclitaxel against oral squamous cell carcinoma in nude mice

作　　者：陈德荣 张晓云 王秀珍 任婷远 王荣德 CHEN Derong;ZHANG Xiaoyun;WANG Xiuzhen;REN Tingyuan;WANG Rongde(Department of Pathology,Zhangye People’s Hospital,Hexi College,734000,China;Department of Pathophysiology,Hexi University Medical College)

机构地区：[1]河西学院附属张掖人民医院病理科,734000 [2]河西学院医学院病理生理教研室

出　　处：《实用口腔医学杂志》2020年第6期870-875,共6页Journal of Practical Stomatology

摘　　要：目的:探索橙皮苷(HES)对口腔鳞状细胞癌(OSCC)的抗癌作用及其与紫杉醇(PTX)应用的抗癌效果。方法:CCK-8实验检测细胞的增殖,流式细胞术检测细胞凋亡,Transwell法检测细胞迁移;构建裸鼠移植瘤模型并分为5组(n=5),用NaCl,10 mg/kg HES,10 mg/kg PTX,20 mg/kg PTX和10 mg/kg HES+10 mg/kg PTX处理,测量瘤体体积、质量,计算肿瘤抑制率;HE染色和TUNEL检测细胞凋亡;Western blot实验检测蛋白表达。结果:10μmol/L HES+10μmol/L PTX组中细胞存活率为16.38%,凋亡细胞比例为83.68%,迁移细胞降低65%,与20μmol/L PTX相比具有显著性差异(P<0.05);HES联合PTX肿瘤抑制率为66.17%,HE染色结果和TUNEL染色实验表明HES+PTX组凋亡细胞数量大于2×PTX组(P<0.05)。HES+PTX组与2×PTX组比较MMP-9,N-cadherin及snail蛋白表达明显下调,E-cadherin蛋白表达升高(P<0.05)。结论:HES对OSCCs具有抑制增殖、诱导凋亡作用,和PTX联用能够显著促进OSCC细胞凋亡,抑制细胞迁移,可能发挥协同抗癌作用。Objective:To study the anticancer effects of hesperidin(HES)and its combination anticancer effects with paclitaxel(PTX)against oral squamous cell carcinoma(OSCC)in nude mice.Methods:CCK-8 assay,flow cytometry,transwell method were used to detect the proliferation,apoptosis and migration of Tca8113 cells treated by HES,PTX and their combination respectively.Nude mouse xenograft models were constructed by transplantation of Tca8113 cells into the nude mice and were divide into 5 groups(n=5),treated with NaCl,10 mg/kg HES,10 mg/kg PTX,20 mg/kg PTX and 10 mg/kg HES+10 mg/kg PTX respectively.The tumor volume,mass and tumor inhibition rate were measured.HE staining and TUNEL examination were used to detect cell apoptosis.Western blot was used to detect related protein expression.Results:HES inhibited the proliferation of Tca8113 cells.In the 10μmol/L HES+10μmol/L PTX group,the cell survival rate was 16.38%,the proportion of apoptotic cells was 83.68%,and the cell migration reduced by 65%,compare with those of 20μmol/L PTX group,P<0.05.HES combined with PTX reduced the tumor volume in vivo,and the tumor inhibition rate was 66.17%.The number of apoptotic cells in the HES+PTX group was more that in the 2×PTX group(P<0.05).The expression of MMP-9,N-cadherin and snail protein in HES+PTX group was down-regulated,and the expression of E-cadherin protein was increased,compare with those of 2×PTX group,P<0.05.Conclusion:HES can inhibit the proliferation and induce apoptosis of OSCC cells,and its combination with PTX can promote the apoptosis of OSCC cells,inhibit cell migration,and may play a synergistic role against OSCC.

关 键 词：橙皮苷(HES) 口腔鳞状细胞癌(OSCC) 荷瘤裸鼠 紫杉醇(PTX) 

分 类 号：R739.8[医药卫生—肿瘤]