沉默HIF-1α基因对口腔鳞癌细胞凋亡及周期的影响  

The effects of HIF-1αgene silencing on the apoptosis and cell cycle of oral squamous cell carcinoma

作　　者：许亮[1] 刘姗姗 周小芬 马胜男 朱小峰[1] XU Liang;LIU Shanshan;ZHOU Xiaofen;MA Shengnan;ZHU Xiaofeng(The First Affiliated Hospital of Fujian Medical University,350001 Fuzhou,China)

机构地区：[1]福建医科大学附属第一医院口腔科,福州350001

出　　处：《实用口腔医学杂志》2020年第6期939-943,共5页Journal of Practical Stomatology

摘　　要：目的:探究HIF-1α基因沉默对口腔鳞癌细胞(TSCCA)的增殖、凋亡及周期的影响。方法:实验设置正常对照组、阴性对照组、基因沉默组,利用CCK-8法对细胞增殖率进行检测,流式细胞术对凋亡率及细胞周期进行检测,Western Blot对凋亡及周期蛋白进行检测。结果:HIF-1α基因沉默后,口腔鳞癌细胞增殖率下降,细胞凋亡率上升,并引起G2/M期周期阻滞;并且,细胞内Bcl-2、Cyclin A、Cyclin B、CDK1/2蛋白表达下降;Bax、caspase-3、PARP、p21的蛋白表达上升。结论:沉默HIF-1α基因能够抑制口腔鳞癌细胞的增殖,促使癌细胞发生线粒体依赖性凋亡以及G2/M期周期阻滞。Objective:To study the effects of HIF-1αgene silencing on the proliferation,apoptosis and cell cycle of oral squamous cell carcinoma TSCCA cells.Methods:TSCCA cells were divided into control,negative control and HIF-1αgene silencing groups.Cell proliferation rate was detected by CCK-8 assay,apoptosis rates and cell cycle were detected by flow cytometry,the expression levels of apoptosis and cell cycle proteins were detected by Western Blot.Results:After HIF-1αgene silencing,cell proliferation rate decreased,cell apoptosis rate increased and G2/M cell cycle arrested at G2/M stage.In addition,the expression levels of Bcl-2,Cyclin A,Cyclin B and CDK1/2 were inhibited,the expression levels of Bad,caspase-3,PARP and p21 were promoted.Conclusion:Silencing the HIF-1αgene can inhibit the proliferation of oral squamous cell carcinoma cells,induce mitochondrial-dependent apoptosis,and cause G2/M cell cycle arrest.

关 键 词：HIF-1Α 基因沉默 口腔癌细胞 细胞凋亡 细胞周期 

分 类 号：R739.8[医药卫生—肿瘤]