MiR-425-5p通过调控PTEN促进乳腺癌细胞体外生长和转移的机制  

MiR-425-5p Promotes Growth and Metastasis of Breast Cancer Cells in Vitro by Regulating PTEN

作　　者：张杨蕊[1] 庞霞[1] 黄培[1] 薛金慧 ZHANG Yangrui;PANG Xia;HUANG Pei(The First Affiliated Hospital of Zhengzhou University,Zhengzhou,450000)

机构地区：[1]郑州大学第一附属医院,450000 [2]郑州大学附属郑州中心医院,450000

出　　处：《实用癌症杂志》2020年第12期1943-1949,共7页The Practical Journal of Cancer

摘　　要：目的研究miR-425-5p对乳腺癌细胞体外生长和转移的影响及机制。方法RT-qPCR检测5种乳腺癌细胞株中miR-425-5p的表达水平;miR-425-5p mimic、inhibitor、NC转染至乳腺癌细胞,CCK8法和Transwell小室法检测转染后乳腺癌细胞的增殖和迁移能力,采用生物信息学的方法预测miR-425-5p的靶基因,荧光素酶报告实验验证靶基因,Western blot检测转染miR-425-5p mimic、inhibitor、NC后靶基因的表达水平。检测过表达及低表达PTEN后,miR-425-5p对乳腺癌细胞增殖和迁移的影响。结果5种乳腺癌细胞株中miR-425-5p表达水平均显著高于乳腺上皮细胞(P<0.05)。miR-425-5p mimic显著促进MCF-7细胞的增殖(P<0.05)和迁移能力(P<0.001);miR-425-5p inhibitor显著抑制MDA-MB-231细胞的增殖(P<0.05)和迁移能力(P<0.001)。预测并验证PTEN为miR-425-5p的靶基因,miR-425-5p能够下调PTEN的表达;过表达PTEN后,miR-425-5p mimic的促增殖和迁移作用则被显著抑制(P<0.05);抑制PTEN表达后miR-425-5p inhibitor的抑制增殖和迁移作用亦被显著增强(P<0.05)。结论miR-425-5p能够调控乳腺癌细胞的体外生长和转移,其机制可能为抑制抑癌基因PTEN的表达。Objective To study the effect and mechanism of miR-425-5p on breast cancer cell growth and metastasis in vitro.Methods RT-qPCR was used to detect the expression level of miR-425-5p in 5 breast cancer cell lines;miR-425-5p mimic,inhibitor,and NC were transfected into breast cancer cells.CCK8 method was used to detect breast cancer cells after transfection.Proliferation and migration ability,bioinformatics method was used to predict the target gene of miR-425-5p,luciferase report experiment was used to verify the target gene,and Western blot was used to detect the transfection of miR-425-The expression levels of target genes after 5p mimic,inhibitor,and NC;the effects of miR-425-5p on breast cancer cell proliferation and migration were detected after overexpression and low expression of PTEN.Results The expression levels of miR-425-5p in the five breast cancer cell lines were significantly higher than those in breast epithelial cells(P<0.05);miR-425-5p mimic significantly promoted the proliferation(P<0.05)and migration ability of MCF-7 cells.(P<0.001);miR-425-5p inhibitor significantly inhibited MDA-MB-231 cell proliferation(P<0.05)and migration ability(P<0.001);PTEN was predicted and verified as a target gene of miR-425-5p,miR-425-5p can down-regulate PTEN expression;after PTEN overexpression,miR-425-5p mimic's pro-proliferation and migration effects were significantly inhibition(P<0.05);the inhibition of miR-425-5p inhibitor's proliferation and migration was also significantly inhibited after inhibiting PTEN expression(P<0.05).Conclusion miR-425-5p can regulate the growth and metastasis of breast cancer cells in vitro,and its mechanism may be the inhibition of the expression of tumor suppressor gene PTEN.

关 键 词：miR-425-5p 乳腺癌 生长 转移 

分 类 号：R737.9[医药卫生—肿瘤]