ChIP-seq技术的研究进展  

Research Progress of ChIP-Seq Technology

作　　者：施威扬 袁明波 SHI Wei-Yang;YUAN Ming-Bo(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China)

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2021年第2期46-52,62,共8页Periodical of Ocean University of China

基　　金：国家重点研究发展计划项目(2018YFD0900604);国家自然科学基金项目(41676119,41476120)资助。

摘　　要：染色体免疫共沉淀测序(Chromatin immunoprecipitation followed by sequencing,ChIP-seq)是研究DNA-蛋白质互作的有力工具,被广泛用于RNA聚合酶、转录因子和组蛋白修饰等在基因组上的精确定位。近年来,在ChIP-seq技术的基础上,科学家提出了一系列研究DNA-蛋白质互作的技术方法,提高了测序分辨率,降低了实验成本,极大推动了表观基因组学的发展。本文综述了多种DNA-蛋白质互作研究技术的原理及其应用场景,介绍了在单细胞水平上研究DNA-蛋白质互作的实现方法,并展望其未来发展的方向。Chromatin immunoprecipitation followed by sequencing(ChIP-seq)is a powerful tool for studying DNA-protein interactions,which is a widely used method for mapping histone modifications,transcription factors and other protein-DNA interactions genome-wide.In recent years,on the basis of ChIP-seq technology,scientists have proposed a series of new technical methods to study DNA-protein interactions,which has improved sequencing resolution,reduced experimental costs,and greatly promoted the development of epigenomics.This article reviews the related technologies of studying DNA-protein interactions and their applications in biological research,introduces the study of DNA-protein interactions at the single-cell level and its future development direction.

关 键 词：DNA-蛋白质互作 CHIP-SEQ CUT&RUN CUT&Tag 单细胞 

分 类 号：P735[天文地球—海洋生物学]