视网膜Müller细胞中谷氨酸受体2的表达分析  

Expression of glutamate receptor 2 in retinal Müller cells

作　　者：杨梅 胡秋梅 黄艳明 YANG Mei;HU Qiu-mei;HUANG Yan-ming(Department of Ophthalmology,Second Affliated Hospital of Army Medical University,Chongqing 400037,China;Department of Ophthalmology,Daping Hospital Affiliated to Army Medical University,Chongqing 400042,China)

机构地区：[1]陆军军医大学第二附属医院眼科,重庆400037 [2]陆军军医大学大坪医院眼科,重庆400042

出　　处：《局解手术学杂志》2020年第12期941-944,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金项目(81600758)。

摘　　要：目的明确谷氨酸受体2(GluR2)在大鼠视网膜Müller细胞中的表达情况,为视网膜胶质细胞的神经保护作用提供新的研究靶点。方法采用新生(1~2 d)的SD大鼠视网膜组织原代培养Müller细胞,通过免疫荧光染色Vimentin鉴定传代培养的Müller细胞纯度;取新生(1~2 d)的SD大鼠大脑皮质作为阳性对照组,Müller细胞作为实验组。采用Real-Time PCR和Western blot检测2组中GluR2 mRNA和蛋白表达情况。结果传代培养到第3代的Müller细胞纯度达95%;阳性对照组GluR2的mRNA表达水平为(100±17.34)%,蛋白表达水平为(100±14.28)%;实验组GluR2的mRNA表达水平为(86.47±19.23)%,蛋白表达水平为(82.82±11.34)%。结论体外培养的视网膜Müller细胞中有GluR2的mRNA和蛋白表达。Objective To explore the expression of Glutamate receptors 2(GluR2)in the Müller cells of the rat retina,providing a new research target for the neuroprotective effect of retinal glial cells.Methods Retinal tissues of Sprague Dawley(SD)rats born 1 to 2 days were used for the primary cell culture of Müller cells and Immunofluorescence staining was used to identify the purity of subcultured Müller cells with Vimentin.The cerebral cortices of SD rats born 1 to 2 days were taken as the positive control group,and Müller cells were taken as the experimental group.Real-Time polymerase chain reaction(PCR)and Western blot were used to detect the expression levels of GluR2 mRNA and its protein.Results The purity of the tertiary-culture Müller cells was 95%.GluR2 mRNA and protein in the positive control group were(100±17.34)%and(100±14.28)%,these in the experimental group were(86.47±19.23)%and(82.82±11.34)%respectively.Conclusion GluR2 mRNA and protein were detected in in-vitro cultured retinal Müller cells.

关 键 词：视网膜 神经保护 谷氨酸 神经元 MÜLLER细胞 

分 类 号：R774[医药卫生—眼科]