细胞毒素-1对人卵巢癌细胞体外增殖、凋亡的影响  

Effect of cytotoxin-1 on human ovarian cancer cell proliferation and apoptosis in vitro

作　　者：黄千峰[1] 武丽[1] 欧燕兰[1] 艾君[1] 张秀[1] HUANG Qianfeng;WU Li;OU Yanlan;AI Jun;ZHANG Xiu(Guangdong Women and Children Hospital,Guangzhou 511400,China)

机构地区：[1]广东省妇幼保健院,广州511400

出　　处：《广州医药》2020年第6期24-27,32,共5页Guangzhou Medical Journal

基　　金：中国疾病预防控制中心妇幼保健中心科研项目(2019FYH003)。

摘　　要：目的探讨细胞毒素-1(Cytotoxin-1,CTX-1)对人卵巢癌SKOV-3细胞增殖凋亡的影响。方法利用0、4、8、12μg/mL浓度CTX-1处理SKOV-3细胞6、12、24 h,MTS法检测细胞活性,8μg/mL CTX-1处理SKOV-3细胞24、48 h,Hoechst-33258荧光染色观察细胞核染色质形态。取处理6、12 h后细胞,利用流式细胞仪检测SKOV-3细胞的凋亡率。结果4、8、12μg/mL的CTX-1可抑制SKOV-3细胞活性及增殖,呈时间-剂量依赖。Hoechst-33258染色观察可见细胞染色质呈固缩或碎裂状、染色质着色不均、核形态各异,随时间增加而更趋明显。8μg/mL CTX-1处理细胞,6 h细胞坏死率为(1.90±0.27)%,晚期凋亡率为(10.96±1.56)%,而早期凋亡率为(1.52±0.39)%;12 h细胞坏死率为(10.62±0.96)%,晚期凋亡率(15.07±1.23)%,而早期凋亡率为(1.88±0.17)%,与对照组比较,差异有统计学意义(P<0.01)。结论CTX-1可以抑制人卵巢癌细胞活性、抑制其体外增殖、诱导其发生凋亡,该作用呈剂量依赖和时间依赖,主要引起细胞晚期凋亡和坏死。Objective To investigate the effect of cytotoxin-1(CTX-1)on the proliferation and apoptosis of ovarian cancer SKOV-3 cells.Methods SKOV-3 cells were treated with CTX-1 at concentrations of 0,4,8,12μg/mL for 6,12,and 24 hours respectively.Cell viability was measured by MTS method.SKOV-3 cells were treated with 8μg/mL CTX-1 for 24 and 48 hours,by Hoechst-33258 fluorescence staining to observe the morphology of nuclear chromatin.The apoptotic rate of SKOV-3 cells was detected by flow cytometry after 6 and 12 hours of treatment.Results CTX-1 at 4,8,and 12μg/mL inhibited the activity and proliferation of SKOV-3 cells in a time-dose-dependent manner.Hoechst-33258 staining observation showed that the apoptotic cell chromatin was condensed or fragmented chromatin,the chromatin was unevenly colored,and the nuclear morphology was different.It became more obvious with time.8μg/mL CTX-1 treated cells,the 6 h cell necrosis rate was(1.90±0.27)%,the late apoptosis rate was(10.96±1.56)%,and the early apoptosis rate was(1.52±0.39)%;12 hours cell necrosis rate was(10.62±0.96)%,late apoptosis rate was(15.07±1.23)%,and early apoptosis rate was(1.88±0.17)%,compared with the control group,the difference was statistically significant(P<0.01).Conclusion CTX-1 may inhibit the activity of human ovarian cancer cells,inhibit its proliferation in vitro,and induce its apoptosis.The effect is dose-dependent and time-dependent.Mainly it causes late apoptosis and necrosis of cells.

关 键 词：细胞毒素-1 人卵巢癌SKOV-3细胞 增殖 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]