秦皮甲素对高糖诱导肾小球系膜细胞的保护作用与相关炎症机制研究  

Protective effects of Esculin on glomerular mesangial cells exposed to high glucose and its related inflammatory mechanisms

作　　者：姚红月 刘春娜 刘新宇 YAO Hongyue;LIU Chunna;LIU Xinyu(Department of Endocrinology,the First Hospital Affiliated to Jinzhou Medical University,Jinzhou,Liaoning 121001,China;Department of Pharmacology,Jinzhou Medical University,Jinzhou,Liaoning 121001,China)

机构地区：[1]锦州医科大学附属第一医院内分泌科,辽宁锦州121001 [2]锦州医科大学药理学教研室,辽宁锦州121001

出　　处：《重庆医学》2020年第23期3871-3875,共5页Chongqing medicine

基　　金：辽宁省重点研发计划指导计划项目(2018225030);辽宁省自然基金指导计划项目(2019-ZD-0822,2019-ZD-0821)。

摘　　要：目的探讨秦皮甲素(ESCL)对高糖(HG)诱导肾小球系膜细胞(GMCs)的影响,以及ESCL对糖尿病肾病(DNP)的保护作用与相关炎症机制。方法选取GMCs建立DNP细胞模型,将细胞分为5组:NG组、HG组、ESCL-L、ESCL-M组和ESCL-H组。观察不同浓度ESCL对HG诱导GMCs总蛋白含量、体积和生存率的影响,并测定各组白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及基质细胞衍生因子-1(SDF-1)的水平,采用Western blot方法检测其作用是否通过影响蛋白激酶B(PKB)途径而实现。结果与NG组比较,HG诱导的GMCs数量减少、存活率下降、体积增大、总蛋白含量增加,差异有统计学意义(P<0.05)。与HG组相比,经不同浓度ESCL处理后的GMCs数量增多、体积缩小、存活率升高、总蛋白含量降低,差异有统计学意义(P<0.05)。与HG组相比,不同浓度ESCL能降低IL-6、TNF-α和SDF-1水平,升高p-Akt并降低Akt表达,差异有统计学意义(P<0.05)。结论ESCL可能是通过影响p-Akt-Akt信号通路,下调多种相关炎症因子和炎性趋化因子的表达,对DNP具有一定保护作用。Objective To investigate the effects of esculin(ESCL)on glomerular mesangial cells(GMCs)exposed to high glucose(HG).To investigate the protective effect of ESCL on diabetic nephropathy(DNP)and its associated inflammatory.Methods GMCs were used for establishe DNP cell model and divided into 5 groups,included the NG group,the HG group,the ESCL-L group,the ESCL-M group and the ESCL-H group.The total protein content,volume and survival rate of GMCs exposed to HG by different concentrations of ESCL were observed.The contents of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and stromal cell derived factor-1(SDF-1).In addition,the expression of protein kinase B(PKB)was tested by Western blot.Results Compared with the NG group,the amount of GMCs decreased in the HG group,the survival rate decreased,the volume and total protein content increased(P<0.05).Compared with the HG group,the amount of GMCs and survival rate increased,and volume of GMCs and the total protein content decreased by different concentration of ESCL(P<0.05).Compared with the HG group,the contents of SDF-1,IL-6 and TNF-αdecreased by different concentration of ESCL,by which also increased the expression of p-Akt and decreased Akt(P<0.05).Conclusion ESCL could protect GMCs from DNP injury through regulating p-Akt-Akt pathway,inhibiting inflammatory factors and chemokines.

关 键 词：秦皮甲素 糖尿病肾病 肾小球系膜细胞 肿瘤坏死因子Α 白细胞介素6 基质细胞衍生因子-1 

分 类 号：R575.2[医药卫生—消化系统]