红背叶根对乙醇诱导的酒精性脂肪肝细胞模型的影响  

Effects of Radix Alchorneae Trewioid on alcoholic fatty liver cell model induced by ethanol

作　　者：沈海容 王大东[1] 罗显克[2] 王保健[2] 吴晓莉[2] 农云翠 SHEN Hairong;WANG Dadong;LUO Xianke;WANG Baojian;WU Xiaoli;NONG Yuncui(Department of Gastroenterology Second Ward,National Hospital of Guangxi Zhuang Autonomous Region,Nanning,Guangxi 530000,China;Department of Gastroenterology First Ward,National Hospital of Guangxi Zhuang Autonomous Region,Nanning,Guangxi 530000,China)

机构地区：[1]广西壮族自治区民族医院消化内科二区,广西南宁530000 [2]广西壮族自治区民族医院消化内科一区,广西南宁530000

出　　处：《检验医学与临床》2020年第24期3561-3564,共4页Laboratory Medicine and Clinic

基　　金：广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z20180610)。

摘　　要：目的研究红背叶根对乙醇诱导的酒精性脂肪肝细胞模型的影响。方法采用乙醇诱导建立酒精性脂肪肝的L02细胞模型,然后用不同水平红背叶根水提物处理该细胞模型(红背叶根组),设立对照组(未使用乙醇诱导的L02细胞)与模型组(80 mmol/L乙醇诱导的酒精性脂肪肝L02细胞模型)。检测各组细胞上清液中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、γ-谷氨酰转移酶(GGT)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平。结果随着乙醇水平升高,L02细胞的存活率逐渐下降,差异均有统计学意义(P<0.05)。红背叶根组中,随着红背叶根水提物水平逐渐降低,酒精性脂肪肝L02细胞模型中细胞存活率逐渐升高,差异均有统计学意义(P<0.05)。与对照组比较,模型组酒精性脂肪肝L02细胞模型的细胞上清液中ALT、AST、GGT、MDA水平明显升高,SOD水平明显降低,差异均有统计学意义(P<0.05)。与模型组比较,红背叶根组3个水平下,酒精性脂肪肝L02细胞模型的细胞上清液中ALT、AST、GGT、MDA水平明显降低,SOD水平明显升高,差异均有统计学意义(P<0.05)。结论红背叶根在酒精性脂肪肝细胞模型中具有保肝、降酶、抗脂质过氧化的作用。Objective To study the effect of Radix Alchorneae Trewioid on alcoholic fatty liver cell model induced by ethanol.Methods L02 cell model of alcoholic fatty liver was established by ethanol induction.Then,the cell model was treated with different levels of aqueous extract of Radix Alchorneae Trewioid(Radix Alchorneae Trewioid group).The control group(L02 cell not induced by ethanol)and model group(L02 cell model of alcoholic fatty liver induced by 80 mmol/L ethanol)were established.The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),γ-glutamyltransferase(GGT),superoxide dismutase(SOD)and malondialdehyde(MDA)in the cell supernatant of each group were detected.Results With the increase of ethanol level,the survival rate of L02 cell decreased gradually,and the differences were statistically significant(P<0.05).In the Radix Alchorneae Trewioid group,the cell survival rate in the alcoholic fatty liver L02 cell model increased gradually with the decrease of aqueous extract of Radix Alchorneae Trewioid levels,and the differences were statistically significant(P<0.05).Compared with the control group,the ALT,AST,GGT and MDA levels in the cell supernatant of the alcoholic fatty liver L02 cell model in the model group were significantly increased,the SOD level was significantly decreased,and the differences were statistically significant(P<0.05).Compared with the model group,at 3 levels of the Radix Alchorneae Trewioid group,the ALT,AST,GGT and MDA levels in the cell supernatant of the alcoholic fatty liver L02 cell model were significantly reduced,the SOD levels were significantly increased,and the differences were statistically significant(P<0.05).Conclusion Radix Alchorneae Trewioid can protect the liver,lower enzymes and resist lipid peroxidation in alcoholic fatty liver cell model.

关 键 词：红背叶根 L02细胞 酒精性脂肪肝 

分 类 号：R575.5[医药卫生—消化系统]