人血清白蛋白Langmuir-Blodgett薄膜的制备及表征  

Preparation and Characterization of Human Serum Albumin Langmuir-Blodgett Films

作　　者：芦菲[1] Lu Fei(College of Chemical&Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022

出　　处：《广东化工》2020年第22期240-241,共2页Guangdong Chemical Industry

基　　金：2017年吉林市青年科技工作者创新创业大赛(201750253)。

摘　　要：采用Langmuir-Blodget(LB)技术成功制备了人血清白蛋白(HSA)Langmuir单层膜和LB多层膜。表面压力(π)-面积(A)曲线中明显出现了较高的崩溃压力和成膜曲线。研究了pH对HSA Langmuir单层膜形成的影响。用布鲁斯特角显微镜(BAM)分析了HSA分子在空气-水界面单层的聚集现象。ATR结果表明,HSA LB膜中的蛋白质二级结构出现了β旋转角结构。The monolayer of human serum albumin(HSA)was successfully prepared by LB technology.High crash pressure and film forming process is obviously appeared in the surface pressure(π)-area(A)curve.The effects of pH on HSA Langmuir monolayer formation was studied.The characterization of monolayer at the air-water interfaces was analyzed by Brewster angle microscope(BAM).ATR result showed that the secondary structure of HSA changes fromα-helix in solution toβ-rotation angle in LB film.

关 键 词：人血清白蛋白 LANGMUIR-BLODGETT 蛋白质薄膜 

分 类 号：TS207.3[轻工技术与工程—食品科学]