TG-相互作用因子在维甲酸抑制腭突间充质细胞增殖过程中的作用  

Role of TGIF in the inhibition of the proliferation of palatal mesenchymal cells induced by retinoic acid

作　　者：刘小转 高素华[1] 余凯伦 陈瑶 陶宇昌 何志东 余增丽 LIU Xiaozhuan;GAO Suhua;YU Kailun;CHEN Yao;TAO Yuchang;HE Zhidong;YU Zengli(Center for Clinical Single-Cell Biomedicine,Henan Provincial People′s Hospital,Zhengzhou 450003;Department of Nutrition and Food Hygiene,College of Public Health,Zhengzhou University,Zhengzhou 450001)

机构地区：[1]河南省人民医院临床单细胞生物医学中心,郑州450003 [2]郑州大学公共卫生学院营养与食品卫生学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2020年第6期755-759,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(81801547);河南省科技攻关项目(182102310222)。

摘　　要：目的:探索TG-相互作用因子(TGIF)在维甲酸抑制腭突间充质细胞增殖过程中作用。方法:分离、培养妊娠13 d胎鼠腭突间充质细胞。MTT实验检测0~10.0μmol/L全反式维甲酸(atRA)在不同时间点对胎鼠腭突间充质细胞增殖的影响。Western blot检测对照组、5.0μmol/L atRA处理组细胞内TGIF蛋白的表达。TGIF siRNA和control siRNA分别转染胎鼠腭突间充质细胞,并设置空白对照组,Western blot检测TGIF siRNA转染胎鼠腭突间充质细胞后的基因沉默的效果;MTT实验检测TGIF siRNA转染后atRA对胎鼠腭突间充质细胞增殖的影响。结果:0.1~10.0μmol/L的atRA对不同时间点胎鼠腭突间充质细胞的生长均有抑制作用,呈时间-剂量依赖性;且在72 h时,5.0和10.0μmol/L的atRA对胎鼠腭突间充质细胞的增殖具有明显的抑制作用(P<0.05)。TGIF siRNA转染后,5.0μmol/L atRA对胎鼠腭突间充质细胞增殖的抑制作用不明显(P>0.05)。结论:atRA对腭突间充质细胞增殖的抑制作用可能主要通过TGIF介导。Aim:To investigate the role of TG-interacting factor(TGIF)in inhibition of the proliferation of palatal mesenchymal cells induced by retinoic acid.Methods:Mouse embryonic palatal mesenchyme cells(EPMCs)were isolated from gestation day 13 embryos and cultured.The proliferation of EPMC was detected by MTT assay after all-trans retinoic acid(atRA)treatment(ranging from 0 to 10.0μmol/L)for various time.TGIF protein level was detected by Western blot in control group and 5.0μmol/L atRA treatment group.EPMCs were transfected by TGIF siRNA and control siRNA,respectively,and blank control was set.The knockdown effect of TGIF siRNA in EPMCs was detected by Western blot.The proliferation of EPMCs with TGIF gene silencing was detected by MTT assay after 5.0μmol/L atRA treatment.Results:atRA treatment(ranging from 0.1 to 10.0μmol/L)significanly inhibit the proliferation of EPMCs in a time-and dose-dependent manner.Both 5.0 and 10.0μmol/L atRA treatment for 72 hours markedly inhibited proliferation of EPMCs(P<0.05).The inhibition of proliferation of EPMCs induced by 5.0μmol/L atRA was blocked by TGIF gene silencing(P>0.05).Conclusion:The inhibition of proliferation of EPMCs induced by atRA may be primarily mediated by TGIF.

关 键 词：腭裂 维甲酸 腭突间充质细胞 TG-相互作用因子 

分 类 号：R782.2[医药卫生—口腔医学]