过表达CSRP2对人B淋巴瘤细胞Ramos增殖、周期分布、凋亡及CREB表达的影响  

Effects of CSRP2 overexpression on human B lymphoma Ramos cells′proliferation,cell cycle,apoptosis and CREB expression

作　　者：王树娟[1] 范熠 张雨 李梦亚 王冲[1] 刘钰 王伟琼[1] 郝倩倩[1] 刘延方[1] WANG Shujuan;FAN Yi;ZHANG Yu;LI Mengya;WANG Chong;LIU Yu;WANG Weiqiong;HAO Qianqian;LIU Yanfang(Department of Hematology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院血液科,郑州450052

出　　处：《郑州大学学报（医学版）》2020年第6期771-774,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(81800137,U1804191)。

摘　　要：目的:探讨过表达半胱氨酸和甘氨酸富集蛋白2(CSRP2)对人B淋巴瘤细胞Ramos增殖、周期分布、凋亡及CREB表达的影响。方法:通过慢病毒感染的方法构建CSRP2过表达的淋巴瘤Ramos细胞模型(过表达组),以感染对照慢病毒的Ramos细胞为对照。采用RT-PCR和Western blot检测感染效率。采用CCK-8法检测2组细胞增殖情况,采用流式细胞术和Annexin V/PI双染法分别检测细胞周期分布和凋亡,并用Western blot检测CREB的表达水平。结果:与对照组比较,过表达组CSRP2 mRNA和蛋白表达水平升高(P<0.05)。CSRP2过表达可促进细胞增殖和细胞周期进展(P<0.05),但对细胞凋亡无影响(P>0.05)。CSRP2过表达可显著上调CREB的磷酸化水平(P<0.05)。结论:CSRP2可能通过上调CREB的表达促进淋巴瘤细胞增殖。Aim:To explore the effects of CSRP2 gene on the proliferation,cell cycle,apoptosis and CREB expression of human B lymphoma cell line Ramos.Methods:A CSRP2 overexpressing lymphoma cell model was constructed by lentivirus infection(overexpression group).The expression of CSRP2 in the 2 groups was verified by RT-PCR and Western blot;cell proliferation was detected by CCK-8 assay,and cell cycle and cell apoptosis were detected by flow cytometry and Annexin V/PI double staining.Western blot was used to detect the expression of CREB.Results:CSRP2 overexpressing lymphoma cell line was successfully constructed.CSRP2 overexpression could promote lymphoma cell line proliferation and cell cycle progression(P<0.05),but had no effect on apoptosis(P>0.05).CSRP2 overexpression could significantly increase the phosphorylation level of CREB(P<0.05).Conclusion:CSRP2 may promote lymphoma cell proliferation by upregulating the expression of CREB.

关 键 词：CSRP2 淋巴瘤 CREB信号通路 增殖 细胞周期 凋亡 

分 类 号：R733[医药卫生—肿瘤]