肝癌免疫相关预后标志物的分析  

Analysis of immune-related prognostic markers of liver cancer

作　　者：鞠铭伊 毕佳 卫倩 江龙洋 齐傲霜 宋昕樾 关秋彤 张明 赵琳 魏敏杰 孙明立 JU Mingyi;BI Jia;WEI Qian;JIANG Longyang;QI Aoshuang;SONG Xinyue;GUAN Qiutong;ZHANG Ming;ZHAO Lin;WEI Minjie;SUN Mingli(Department of Pharmacology,School of Pharmacy China Medical University,Liaoning Key Laboratory of Molecular Targeted Anti-tumor Drug Development and Evaluation,Liaoning Cancer Immune Peptide Drug Engineering Technology Research Center,Shenyang 110122)

机构地区：[1]中国医科大学药学院药理学教研室,辽宁省分子靶向抗肿瘤药物药理学研究与评价实验室,辽宁省肿瘤免疫多肽药物工程研究中心,沈阳110122

出　　处：《郑州大学学报（医学版）》2020年第6期779-785,共7页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(U1608281);沈阳市科技计划项目(19-109-4-09)。

摘　　要：目的:筛选与免疫相关的、稳定的肝癌预后标志物。方法:从癌症基因组图谱(TCGA)数据库中下载肝癌患者的相关数据,筛选出差异表达的肝癌免疫相关基因。通过对这些基因进行单因素、多因素分析,筛选肝癌预后标志物。用DAVID在线工具进行GO和KEGG富集途径分析。利用HPA数据库和GSE14520对结果进行验证。结果:本研究获得了一个由9个与肝癌免疫相关的基因(PSMD14、STC2、SPP1、ISG20L2、MAPT、NR6A1、PSME3、HSP90AA1和CSPG5)组成的肝癌预后标志物,这一标志物与肝癌患者的总生存期显著相关(P<0.001)。结论:由PSMD14、STC2、SPP1、ISG20L2、MAPT、NR6A1、PSME3、HSP90AA1和CSPG5组成的标志物可作为评估肝癌预后的分子标志物,为肝癌的有效免疫治疗策略提供了新的见解。Aim:To mine a prognostic biomarker consisted of immune-related genes for liver cancer.Methods:We selected immune-related differentially expressed genes from gene expression data of liver cancer patients in the cancer genome atlas(TCGA)database.To excavate prognosis biomarkers,we used univariate and multivariate Cox regression analysis.Visualization andintegrated discovery(DAVID)database was used to enrich biological themes on GO terms and on the Kyoto encyclopedia of genes and genomes(KEGG)pathway maps.Human protein atlas(HPA)database and GSE14520 were utilized to testify the stable of our results.Results:In our study,we identified and validated a biomarker consisted of 9 immune-related genes(PSMD14,STC2,SPP1,ISG20L2,MAPT,NR6A1,PSME3,HSP90AA1 and CSPG5)that could be useful for outlining overall survival(OS)of patients with liver cancer(P<0.001).Conclusion:The biomarker consisted of PSMD14,STC2,SPP1,ISG20L2,MAPT,NR6A1,PSME3,HSP90AA1 and CSPG5 is significantly associated with prognosis of liver cancer.Our findings have given novel insights of new immunotherapies for liver cancer.

关 键 词：癌症基因组图谱 基因表达汇编 人类蛋白质图谱 肝癌 免疫 预后标志物 

分 类 号：R735.7[医药卫生—肿瘤]