内皮素受体A对牙周膜干细胞骨向分化能力影响的研究  

Effects of endothelin receptor A on osteoblastic differentiation function of periodontal ligament stem cells

作　　者：梁莉[1] 许亦权[1] 杨楠[1] 王辰 LIANG Li;XU Yiquan;YANG Nan;WANG Chen(Department of Stomatology,the Eighth Medical Center,Chinese PLA General Hospital,Beijing 100091,China)

机构地区：[1]解放军总医院第八医学中心口腔科,北京100091

出　　处：《口腔生物医学》2020年第3期161-163,共3页Oral Biomedicine

基　　金：国家自然科学基金(81500821)。

摘　　要：目的:研究内皮素受体A(ETRA)对牙周膜干细胞(PDLSCs)成骨分化能力的影响。方法:构建ETRA小干扰RNA(siRNA)载体,转染PDLSCs后,采用实时定量RT-PCR及Western Blot检测转染前后ETRA的表达情况。用100 nmol/L内皮素-1(ET-1)刺激siETRA转染和未转染的的PDLSCs 14 d,碱性磷酸酶(ALP)染色观察PDLSCs成骨能力的变化,实时定量RT-PCR及Western Blot检测PDLSCs Runt相关转录因子2(RUNX2)和骨钙素(OCN)的表达情况。结果:ETRA干扰载体可有效地抑制PDLSCs中ETRA表达。ET-1刺激后,未转染组PDLSCs ALP染色增强,RUNX2和OCN表达升高(P<0.05),siETRA稳定转染组无显著变化(P>0.05)。结论:ET-1可能通过ETRA促进PDLSCs的骨向分化能力。Objective:To investigate the effects of endothelin receptor A(ETRA)on osteogenic differentiation function of periodontal ligament stem cells(PDLSCs).Methods:The small interfering RNA(siRNA)technique was employed to inhibit ETRA expression in PDLSCs and cells were cultured with ET-1(100 nmol/L)for 14 days.The osteoblastic differentiation function of PDLSCs was observed by alkaline phosphatase staining.The expression of RUNX2 and OCN mRNAs and proteins in PDLSCs were detected by real-time RT-PCR and Western Blot,respectively.Results:ETRA interference vector can effectively inhibit the expression of ETRA in PDLSCs.ET-1 significantly enhanced the expression of RUNX2 and OCN of PDLSCs(P<0.05),However,ET-1 had no effect on the expression of RUNX2 and OCN of PDLSCs stably transfected by siETRA(P>0.05).Conclusions:These results indicate that endothelin-1 may stimulate the osteogenic differentiation of PDLSCs via ETRA.

关 键 词：内皮素受体A 牙周膜干细胞 成骨分化 小干扰RNA 

分 类 号：R781.4[医药卫生—口腔医学]