不同正畸力值对哺乳期大鼠正畸牙周组织中HO-1 CC类趋化因子受体1及其配体的影响  

Effect of different orthodontic force values on HO-1,CC chemokine receptor 1 and their ligands in periodontal tissues of lactating rats

作　　者：焉宏军 王荣强 张雯姝 YAN Hongjun;WANG Rongqiang;ZHANG Wenshu(Department of Orthodontics,Dandong Central Hospital Stomatology District(former Dandong Stomatological Hospital),Dandong 118000,China)

机构地区：[1]辽宁省丹东市中心医院口腔院区(原丹东市口腔医院)口腔正畸科,118000

出　　处：《安徽医学》2020年第11期1249-1253,共5页Anhui Medical Journal

基　　金：国家重点研发计划(项目编号:2016YFC0106604)。

摘　　要：目的探讨不同正畸力值对哺乳期大鼠正畸牙周组织中血红素氧合酶(HO-1)、CC类趋化因子受体1及其配体的影响。方法选择3月龄Wistar大鼠126只制备哺乳期大鼠模型,成功制备72只,按随机数字表法分为0N组、0.29N组、0.49N组及0.98N组,每组18只。每组依次给予0N、0.29N、0.49N及0.98N的正畸力,比较干预前1天及干预第1、3、7天后4组大鼠的CC趋化因子受体1(CCR1)及其配体(CCL3、CCL5)mRNA相对表达量、HO-1表达及抗酒石酸酸性磷酸酶(TRAP)破骨细胞染色阳性面积。结果干预第1、3天,0.29 N组、0.49 N组及0.98 N组CCR1、CCR3和CCL5 mRNA表达水平及破骨细胞染色阳性面积较0N组均明显增加(P<0.05),0.49N组及0.98N组上述指标水平较0.29N组均明显增加(P<0.05),而0.49N组与0.98N组这些指标比较,差异均无统计学意义(P>0.05)。结论正畸干预可通过促进哺乳期大鼠牙周组织中HO-1、CCR1及其配体的表达发挥作用。Objective To investigate the effects of different orthodontic force values on HO-1,CC chemokine receptor 1 and their ligands in the periodontal tissues of lactating rats.Methods One hundred and twenty-six3-month-old Wistar rats were selected to prepare lactating rat models,among which 72 rats were successfully prepared.According to the random number table method,they were divided into ON group,0.29 N group,0.49 N group and 0.98 N group,with 18 ratsin each.Each group was given 0 N,0.29 N,0.49 N and 0.98 N orthodontic force in turn,and the relative expression levels of CC chemokine receptor 1(CCR1)and its ligands(CCL3,CCL5)mRNA,HO-1 expression and TRAP osteoclast staining level were compared.Results On1 st,3 rd day of the intervention,the CCR1,CCR3 and CCL5 mRNA expression and osteoclast levels in 0.29 N,0.49 N and 0.98 N groups were significantly higher than those in 0 N group,and the difference was statistically significant(P<0.05).In0.49 N group and 0.98 N group,CCR1,CCR3 and CCL5 mRNA expression and osteoclast levels were significantly up-regulated compared with those in0.29 N group,andthe difference was statistically significant(P<0.05),while there was no statistically significant difference in this index between 0.49 N group and 0.98 N group(P>0.05).Conclusions Orthodontic intervention can play a therapeutic role by promoting the expression of HO-1,CC chemokine receptor 1 and their ligands in the periodontal tissues of lactating rats.In this study,0.49 N is the most suitable value of orthodontic force for lactating rats,and 3 days of intervention is the most suitable duration of intervention.

关 键 词：正畸力值 哺乳期大鼠模型 牙周组织 血红素氧合酶 CC类趋化因子受体1 

分 类 号：R783.5[医药卫生—口腔医学]