喉癌组织中miRNA-384、miRNA-let-7a的表达及临床意义  

Expression and clinical significance of miRNA-384 and miRNA-let-7a in laryngeal cancer

作　　者：侯俊胜 豆彪 陶学勇[1] 翟性友 HOU Junsheng;DOU Biao;TAO Xueyong;ZHAI Xingyou(Department of Otorhinolaryngology Head and Neck Surgery,the First Affiliated Hospital of He’nan University,Kaifeng 475000,He’nan,China;Department of Otorhinolaryngology Head and Neck Surgery,Hainan Hospital of PLA General Hospital,Sanya 572013,Hainan,China)

机构地区：[1]河南大学第一附属医院耳鼻咽喉头颈外科,河南开封475000 [2]解放军总医院海南医院耳鼻咽喉头颈外科,海南三亚572013

出　　处：《癌症进展》2020年第22期2300-2303,共4页Oncology Progress

摘　　要：目的探讨喉癌组织中微小RNA-384(miRNA-384)、miRNA-let-7a的表达水平及临床意义。方法收集80例喉癌患者的临床资料及随访结果。采用实时荧光定量聚合酶链反应(qRT-PCR)检测喉癌组织及癌旁正常组织中miRNA-384、miRNA-let-7a的相对表达量,研究其与患者临床特征的关系,并采用Kaplan-Meier法分析其表达与患者预后的关系。结果喉癌组织中miRNA-384、miRNA-let-7a相对表达量均明显低于癌旁正常组织,差异均有统计学意义(P﹤0.01)。喉癌组织中miRNA-384相对表达量与miRNA-let-7a相对表达量呈正相关(r=0.457,P﹤0.05)。临床分期为Ⅰ~Ⅱ期、无淋巴结转移的喉癌患者的喉癌组织中miRNA-384、miRNA-let-7a相对表达量分别明显高于临床分期为Ⅲ~Ⅳ期、有淋巴结转移的患者,差异均有统计学意义(P﹤0.01)。miRNA-384高表达患者术后5年生存率为84.3%,明显高于低表达患者的66.2%;miRNA-let-7a高表达患者术后5年生存率为82.9%,明显高于低表达患者的64.8%,差异均有统计学意义(P﹤0.01)。结论miRNA-384、miRNA-let-7a在喉癌组织中的低表达与肿瘤发生、侵袭、转移及预后密切相关,其有望成为喉癌分子靶向治疗的新靶点。Objective To investigate the expression and clinical significance of microRNA-384(miRNA-384)and miRNA-let-7a in laryngeal cancer.Method A total of 80 patients with laryngeal cancer were enrolled and their clinical data and follow-up result were collected.The relative expression of miRNA-384 and miRNA-let-7a in laryngeal cancer tissues and adjacent normal tissues were detected by real-time quantitative polymerase chain reaction(qRT-PCR)method,and their relationship with clinical features was also analyzed.Kaplan-Meier method was performed to analyze the relationship between their expression and prognosis of the patients.Result The relative expression of miRNA-384 and miRNA-let-7a in laryngeal cancer tissues were significantly lower than those in normal tissues adjacent to cancer,and the differences were statistically significant(P<0.01).The relative expression of miRNA-384 in laryngeal cancer tissues presented a positive correlation with the relative expression of miRNA-let-7a(r=0.457,P<0.05).The relative expression of miRNA-384 and miRNA-let-7a in laryngeal cancer tissues from patients with laryngeal cancer with clinical stages I-II and no lymph node metastasis were significantly higher than those of patients with clinical stages III-IV and lymph node metastasis,the differences were statistically significant(P<0.01).The 5-year overall survival of patients with high miRNA-384 expression was 84.3%,which was significantly higher than the 66.2%of patients with low expression;the 5-year overall survival of patients with high miRNA-let-7a expression was 82.9%,which was significantly higher than that of patients with low expression whose value was 64.8%,and the differences were statistically significant(P<0.01).Conclusion The low expressions of miRNA-384 and miRNA-let-7a in laryngeal cancer are closely related to tumorigenesis,invasion,metastasis and prognosis,and these indicators promise to become a new target for molecular targeted therapy of laryngeal cancer.

关 键 词：喉癌 miRNA-384 miRNA-let-7a 实时荧光定量聚合酶链反应 

分 类 号：R739.65[医药卫生—肿瘤]