锌胁迫下甘蓝型油菜发芽期下胚轴长的全基因组关联分析  

Genome-wide association analysis reveals zinc-tolerant loci of rapeseed at germination stage

作　　者：魏丽娟[1] 申树林 黄小虎 马国强 王曦彤 杨怡玲 李洹东 王书贤 朱美晨 唐章林[1] 卢坤[1] 李加纳[1] 曲存民[1] WEI Li-Juan;SHEN Shu-Lin;HUANG Xiao-Hu;MA Guo-Qiang;WANG Xi-Tong;YANG Yi-Ling;LI Huan-Dong;WANG Shu-Xian;ZHU Mei-Chen;TANG Zhang-Lin;LU Kun;LI Jia-Na;QU Cun-Min(College of Agronomy and Biotechnology,Southwest University/Chongqing Engineering Research Center for Rapeseed/Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学农学与生物科技学院/油菜工程研究中心/西南大学现代农业科学研究院,重庆400715

出　　处：《作物学报》2021年第2期262-274,共13页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31701460);中央高校基本科研业务费(XDJK2019C041,XDJK2020D023);重庆市基础科学与创新研究项目(cstc2016shms-ztzx80010,cstc2017jcyjAX0321);国家现代农业产业技术体系建设专项(CARS-12);高等学校学科创新引智基地111项目(B12006)资助

摘　　要：锌(Zn)是重要的微量元素之一,但土壤中过量的锌累积会影响植物的生长发育。本研究以不同遗传来源的140份甘蓝型油菜为材料,利用芸薹属60K SNP芯片对锌胁迫下(30 mg L-1)甘蓝型油菜发芽期相对下胚轴长(RHL)进行全基因组关联分析,筛选与甘蓝型油菜发芽期下胚轴长度显著关联的SNP位点及候选基因。群体结构分析表明,供试的140份甘蓝型油菜被分为2个亚群,其中89%材料间亲缘关系小于0.1,说明供试群体材料亲缘关系比较远。GWAS分析共检测到8个与RHL显著关联的SNP位点,单个SNP位点分别可解释22.0%~33.2%的表型变异。转录组分析获得的差异基因GO富集分析结果表明,上调表达基因主要参与氧化还原反应、离子转运、胁迫反应、防御反应和硫化合物转运。综合全基因组关联分析和转录组测序结果,共鉴定到19个与锌胁迫相关的候选基因,包括编码锌指蛋白家族成员(B-box型和ZFP1)、谷胱甘肽转移酶GSTU21、过氧化物酶家族蛋白、ABC和MFS转运蛋白及细胞壁相关激酶蛋白和一些重要的转录因子(BnaA07g27330D、BnaA02g30270D、BnaA07g27840D、BnaA07g31860D和BnaA07g28000),为深入解析油菜锌胁迫分子机制提供了参考。Zinc(Zn)is one of the important mircroelements,but the excessive amount application would affect plant growth and development.Genome-wide association analysis(GWAS)was performed on the relative hypocotyl length(RHL)using the 140 B.napus genotyped under zinc stress treatment(30 mg L^-1)at germination stage by Illumina 60K SNP array,and then significant SNP locus and candidate genes were detected.In the study,the population structure analysis revealed that the 140 B.napus were classified into two subgroups,and the kinship coefficients of the 89%materials were less than 0.1,indicating the tested population had a distant relationship.GWAS analysis indicated that there were significantly 8 SNP locus correlated to RHL,and single SNP loci could give an explanation on the 22.0%–33.2%phenotype variation.The differential expressed gene(DEGs)were detected by RNA-Seq.GO enrichment analysis indicated that the up-regulated genes mainly participated in redox reaction,ion transport,stress response,defense response and sulfur compound transport.Nineteen candidate genes response to zinc stress were identified by GWAS analysis and RNA-seq,including the genes encoding zinc finger protein(B-box type and ZFP1),glutathione transferase GSTU21,peroxidase family protein,ABC and MFS transporters,cell wall-related kinase protein,and genes encoding transcription factors(TF),such as BnaA07g27330D(MYB),BnaA02g30270D(bHLH),BnaA07g27840D(WRKY57),BnaA07g31860D(ORA47),and BnaA07g28000(NAC).This study laid the foundation for understanding the molecular mechanism of zinc stress in B.napus.

关 键 词：甘蓝型油菜 重金属 锌胁迫 全基因组关联分析 发芽期 

分 类 号：X503.231[环境科学与工程—环境工程] S565.4[农业科学—作物学]