生长抑素联合吉西他滨对人胰腺癌BxPC-3细胞增殖抑制作用的研究  

Inhibitory Effect of Somatostatin combined with Gemcitabine on Proliferation of Human Pancreatic Cancer BxPC-3 Cells

作　　者：刘力[1] 王文婷[1] LIU Li;WANG Wenting(Affiliated Hospital of Xiangnan University,Chenzhou City,Hu’nan Province 423000)

机构地区：[1]湘南学院附属医院,湖南省郴州市423000

出　　处：《医学理论与实践》2020年第24期4053-4055,共3页The Journal of Medical Theory and Practice

基　　金：郴州市科技局科研项目(jsyf2017019);湖南省教育厅资助科研项目(16C1487)。

摘　　要：目的:初步探讨生长抑素(SST)、吉西他滨(Gem)单药或联合用药对人原位胰腺癌细胞BxPC-3细胞增殖的影响。方法:(1)体外培养人原位胰腺癌细胞BxPC-3。使用不同浓度生长抑素干预24h、48h、72h;不同浓度吉西他滨干预72h;计算出生长抑素及吉西他滨半数抑制浓度(IC50),联合用药进行干预。具体分组:A组:空白对照组,B组:生长抑素IC50浓度,C组:吉西他滨IC50浓度,D组:1/2生长抑素IC50浓度+1/2吉西他滨IC50浓度,干预72h;(2)MTT法检测各浓度药物对细胞增殖抑制的影响。结果:生长抑素及吉西他滨均可抑制人原位胰腺癌细胞BxPC-3的增殖,且与药物的浓度和时间正相关;联合用药组比单药组的抑制作用明显增强。结论:生长抑素及吉西他滨均可抑制人原位胰腺癌细胞BxPC-3的增殖,两者联合作用效应更强。Objective:Study the effect of somatostatin and gemcitabine alone or combination on the proliferation inhibition of Human pancreatic carcinoma cell line BxPC-3.Methods:(1)Culture of human pancreatic cancer BxPC-3 cells in vitro.Different concentrations of somatostatin were used for 24 hours,48 hours and 72 hours,different concentrations of gemcitabine were used for 72 hours,and half inhibitory concentrations of somatostatin and gemcitabine(IC50)were calculated,Grouping:A group:control group,B group:SST IC50 concentration,C group:Gem IC50 concentration,D group:1/2 SST IC50 Gem concentration+1/2 Gem IC50 concentration,treatment 72 hours.(2)MTT method was used to detect the inhibitory effect of various concentrations of drugs on cell proliferation.Results:Somatostatin and gemcitabine could inhibit the proliferation of human pancreatic carcinoma cell line BxPC-3 in situ,which was positively correlated with the concentration and time of the drug.Conclusion:Somatostatin combined with gemcitabine can inhibit the proliferation of human pancreatic cancer cell line BxPC-3.

关 键 词：生长抑素 吉西他滨 胰腺癌细胞BxPC-3 

分 类 号：R735.9[医药卫生—肿瘤]