结直肠癌组织中γδT淋巴细胞的表达变化及临床意义  

Expression change and clinical significance ofγδT lymphocytes in colorectal cancer tissues

作　　者：吴彦霖 陈雨琪 李赵继 王若沁 石通国 陈卫昌(指导) WU Yan-Lin;CHEN Yu-Qi;LI Zhao-Ji;WANG Ruo-Qin;SHI Tong-Guo;CHEN Wei-Chang(Department of Gastroenterology,the First Affiliated Hospital of Soochow University,Suzhou 215006,China)

机构地区：[1]苏州大学附属第一医院消化内科,苏州215006 [2]江苏省(科教强卫)胃肠道肿瘤免疫重点实验室,苏州215006 [3]江苏省(高校)临床免疫学重点实验室,苏州215006 [4]苏州市消化道肿瘤免疫重点实验室,苏州215006

出　　处：《中国免疫学杂志》2020年第22期2759-2763,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(81672372)。

摘　　要：目的:探讨结直肠癌(CRC)组织内γδT淋巴细胞的表达变化及临床意义。方法:免疫组化法检测90例CRC组织中γδT淋巴细胞和CD8^+T淋巴细胞表达变化。Wilcoxon秩和检验分析γδT淋巴细胞、CD8^+T淋巴细胞在CRC组织及癌旁组织中的表达差异;卡方检验探讨γδT淋巴细胞、CD8^+T淋巴细胞的表达与CRC患者临床病理参数间的关系;Spearman法分析CRC组织中γδT淋巴细胞与CD8^+T淋巴细胞的相关性;Kaplan-Meier法评估γδT淋巴细胞、CD8^+T淋巴细胞表达与结直肠癌患者预后的关系;COX回归模型探究CRC患者的预后危险因素。结果:CRC组织及癌旁组织中γδT淋巴细胞、CD8^+T淋巴细胞表达差异无统计学意义(P>0.05)。CRC组织内γδT淋巴细胞表达变化与CRC临床分期呈负相关,而与CRC患者年龄、性别、肿瘤大小、N分期无关。CD8^+T淋巴细胞表达与CRC患者年龄、性别、肿瘤大小、N分期、临床分期均无显著相关性(P>0.05)。CRC组织内γδT淋巴细胞表达与CD8^+T淋巴细胞表达呈正相关。γδT淋巴细胞高表达的患者预后更佳,CD8^+T淋巴细胞表达水平与CRC患者预后差异无统计学意义(P>0.05)。COX回归模型分析表明,γδT淋巴细胞是评估CRC预后的独立危险因素。结论:CRC组织中γδT淋巴细胞表达变化与CRC患者预后密切相关,对CRC患者预后评估具有重要价值。Objective:To investigate expression change and clinical significance ofγδT lymphocytes in colorectal cancer(CRC)tissues.Methods:Expression changes ofγδT and CD8^+T lymphocytes in 90 CRC tissues were detected by immunohistochemistry.Wilcoxon test was used to analyze differential expressions ofγδT and CD8^+T lymphocytes in CRC and para-carcinoma tissues.A chi-square test was used to investigate relationship between expression and clinic pathological parameters.Correlation betweenγδT lymphocytes levels and CD8^+T lymphocytes levels in CRC tissues was analyzed by Spearman method.Relation of prognosis of patients with CRC andγδT and CD8^+T lymphocytes were explored by Kaplan-Meier survival analysis.COX regression model was used to analyze prognostic factors of CRC.Results:There was no significant difference in expressions ofγδT lymphocytes and CD8^+T lymphocytes in CRC tissues and para-carcinoma tissues.Expression ofγδT lymphocytes in CRC tissues was negatively correlated with clinical stage of CRC,but not statistically significant with age,sex,tumor size and N stage of CRC.Expression of CD8^+T lymphocytes was not statistically significant age,sex,tumor size,N stage and clinical stage of CRC(P>0.05).There was a positive correlation betweenγδT lymphocytes expression and CD8^+T lymphocytes expression in CRC tissues.Prognosis of patients with high expression ofγδT lymphocytes was better,and not statistically significant in expression of CD8^+T lymphocytes.COX regression model analysis showed thatγδT lymphocytes is an independent factor in prognosis evaluating of CRC.Conclusion:Expression level ofγδT lymphocytes in CRC tissues is closely related to prognosis of CRC patients,which has important value in evaluating prognosis of CRC patients.

关 键 词：结直肠癌 ΓΔT淋巴细胞 CD8+T淋巴细胞 预后 

分 类 号：R735.35[医药卫生—肿瘤]