CpG ODN协同小鼠肝癌细胞裂解物对小鼠原位移植性肝癌的抑制作用研究  

Effect of CpG ODN on assisting TCL from liver cancer cells to induce immune response against orthotopic-transplantated liver cancer in mice

作　　者：孙鹏[2] 李欢欢 王卫芳 窦瑶 周晓晶(指导) SUN Peng;LI Huan-Huan;WANG Wei-Fang;DOU Yao;ZHOU Xiao-Jing(Clinical Medical College,Changchun University of Traditional Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学临床医学院,长春130117 [2]吉林省人民医院消化内科,长春130021

出　　处：《中国免疫学杂志》2020年第23期2842-2845,共4页Chinese Journal of Immunology

基　　金：长春中医药大学2019年度科学研究发展基金(培育基金2019KJ17);国家大学生创新创业训练计划(201810199047)资助。

摘　　要：目的:研究CpG ODN与小鼠肝癌细胞(H22)裂解物(TCL)联用对小鼠原位移植性肝癌的抑制作用及机制初探。方法:制备H22细胞裂解物和建立BALB/c小鼠原位移植性肝癌模型。将模型鼠随机分为四组PBS、TCL、CpG ODN和CpG ODN+TCL,观察各组小鼠肿瘤生长情况及小鼠的生存期。采用细胞毒性淋巴细胞(CTL)杀伤实验观察各组小鼠淋巴细胞对肝癌细胞的杀伤情况。用ELISA方法检测CpG ODN协助TCL诱导Th1型细胞因子分泌水平。结果:与其他组相比CpG ODN+TCL明显抑制了肿瘤生长、延长了荷瘤鼠的生存期(P<0.05)。体内外实验显示,CpG ODN能协同TCL刺激小鼠产生Th1型细胞因子和诱导CTL反应。结论:以CpG ODN为佐剂的TCL具有抑制小鼠原位移植性肝癌的作用,且这种作用可能由于CpG ODN协同TCL诱导小鼠产生的Th1型细胞因子和CTL反应实现的。Objective:To study the effect of CpG ODN on assisting TCL from H22 cells to induce immune response against orthotopic-transplantated liver cancer in mice and to explore the mechanism.Methods:We prepared TCL from H22 cells and built BALB/c mice with orthotopic-transplantated liver cancer.The model mice were injected divided into PBS group,TCL group,CpG ODN group and CpG ODN+TCL group randomly.The mice were monitored for tumor growth and recoding the survival.CTL killing experiment was used to observe the killing of liver cancer cells by lymphocytes in each group.ELISA was used to detect CpG ODN assisting TCL to induce Th1 cytokine secretion level.Results:Compared with other groups,CpG ODN+TCL significantly inhibited the growth of orthotopic transplantation liver cancer cells and prolong survival in mice(P<0.05).In vivo and vitro experiments,CTL response and Th1-biased cytokine also induced by CpG ODN adjuvanted TCL.Conclusion:TCL with CpG ODN as an adjuvant has the effect of inhibiting orthotopic liver cancer in mice,and this effect may be realized by CpG ODN and TCL-induced Th1 cytokine and CTL response in mice.

关 键 词：肿瘤细胞裂解物 免疫刺激性寡聚脱氧核苷酸 肝癌 Th1型免疫反应 

分 类 号：R730.51[医药卫生—肿瘤]