黑龙江省粳稻田间与储藏真菌群落演替规律研究  

Study on Field and Storage Fungal Community Diversity in Japonica Rice of Heilongjiang Province

作　　者：杨焕春 李勇[1] 单宏 李莉莉 兰静 赵琳 孙向东 YANG Huan-chun;LI Yong;SHAN Hong;LI Li-li;LAN Jing;ZHAO Lin;SUN Xiang-dong(Anshun University,Anshun 561000,China;Quality and Safety Institute of Agricultural Products,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]安顺学院,贵州安顺561000 [2]黑龙江省农业科学院农产品质量安全研究所,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2020年第12期86-92,共7页Heilongjiang Agricultural Sciences

基　　金：哈尔滨市优秀学科带头人基金资助项目(2013RFXYJ049,2016RAXYJ085);国家农产品质量安全风险评估重大专项(GJFP2019016);安顺学院博士基金(Asxybsjj201901)。

摘　　要：为促进黑龙江省稻谷合理仓储,保障黑龙江省粳稻品质安全,采用Illumina Hiseq高通量测序方法,于2016年秋季在黑龙江省五常地区分别采收3份新鲜水稻样品、将这些样品仓储1,2和3 a后进行ITS区的高通量测序,分析了物种组成和丰度、Alpha多样性和菌群结构。结果表明:入库储藏3 a后,稻谷样品储藏真菌群落构成演化为子囊菌门(Ascomycota)仍占绝对优势,但比重由94.4%下降为82.1%;散囊菌目(Eurotiales,25.6%)和煤炱目(Capnodiales,20.1%)为优势目;曲霉属(Aspergillus,24.7%)和节担菌属(Wallemia,7.4%)为优势属;帚状曲霉(Aspergillus penicillioides,14.3%)为优势种。不同储藏时间的水稻样品真菌物种组成丰富,真菌种群结构存在一定差异。In order to promote reasonable storage and quality safety of paddy rice in Heilongjiang Province,three freshly harvested rice samples were collected in 2016 and stored for 1,2,and 3 years,then analyzed through high-throughput sequencing of Illumina Hiseq to investigate the composition of storage fungal community.These rice samples were conducted high-throughput sequencing of ITS region,species composition,abundance,alpha diversity,and community structure were also analyzed.The results indicated that through three years storage,the storage fungal community in rice samples changed to as follow:Ascomycota was still the absolute predominant phylum,however the proportion declined from 94.4%to 82.1%;Eurotiales(25.6%)and Capnodiales(20.1%)were the predominant order;Aspergillus(24.7%)and Wallemia(7.4%)were the predominant genus;Aspergillus penicillioides(14.3%)was the predominant specie.Fungal species composition of rice samples stored for different times were abundant and differences of fungal composition structure existed among these rice samples.

关 键 词：粳稻 田间真菌 群落构成 多样性 功能预测 

分 类 号：S511.22[农业科学—作物学]