熊果酸通过BMP-2/Smad4/Wnt/β-catenin信号通路介导对去卵巢大鼠骨量流失的保护作用  

Ursolic acid mediates the protective effect of ursolic acid on bone mass loss in ovariectomized rats through BMP-2/Smad4/Wnt/β-catenin signal pathway

作　　者：李洁[1] 孙雅楠 解光越 李斯[4] LI Jie;SUN Yanan;XIE Guangyue;LI Si(Department of Obstetrics and Gynecology, Worker’s Hospital of Tangshan City, Tangshan 063000;Department of Endocrinology, Tangshan Worker’s Hospital, Tangshan 063000;Department of Orthopedics, Tangshan Worker’s Hospital, Tangshan 063000;Department of Cardiology, Tangshan Worker’s Hospital, Tangshan 063000, China)

机构地区：[1]唐山市工人医院妇产科,河北唐山063000 [2]唐山市工人医院内分泌科,河北唐山063000 [3]唐山市工人医院骨科,河北唐山063000 [4]唐山市工人医院心内科,河北唐山063000

出　　处：《中国骨质疏松杂志》2020年第12期1810-1814,共5页Chinese Journal of Osteoporosis

摘　　要：目的探讨熊果酸(XGS)对去卵巢大鼠骨量流失的影响,并探索可能的机制。方法通过双侧去卵巢建立骨质疏松大鼠模型;随后随机分为假手术组(Sham)、去卵巢组(OVX)以及熊果酸组(XGS),每组10只;其中XGS组大鼠接受熊果酸(100 mg/kg)治疗12周;待治疗检测股骨骨量、骨密度(BMD)、骨矿物质含量(BMC)、骨代谢指标以及BMP-2、Smad4、Wnt 1和β-catenin表达。结果治疗12周后,与OVX组相比,Micro-CT和双能X线结果显示XGS组的大鼠骨小梁微观参数、骨密度和骨矿物质含量得到明显改善。XGS组大鼠BMD、BMC、TV/BV、Tb.N、Tb.Th和Tb.Sp较OVX组明显改善(P<0.05)。血清检测结果和生物力学检测结果显示XGS组的最大载荷和弹性模量均高于OVX组(P<0.05);XGS组大鼠血清ALP、OPD和OCN水平较OVX组明显降低,组间差异有统计学意义(P<0.05)。WB检测显示,和OVX组比较,XGS组BMP-2、Smad4、Wnt1和β-catenin表达水平明显上调,比较差异有统计学意义(P<0.05)。结论熊果酸可以通过BMP-2/Smad4/Wnt/β-catenin信号通路介导对去卵巢大鼠骨量流失保护作用。Objective To investigate the effect of ursolic acid(XGS)on bone mass loss in ovariectomized rats and to explore the possible mechanism.Methods The osteoporotic rat model was established by bilateral ovariectomy.Then the rats were randomly divided into sham operation group(n=10),ovariectomized group(OVX),and XGS group(n=10).The rats in XGS group received XGS(100 mg/kg)for 12 weeks.Bone mass,bone mineral density(BMD),bone mineral content(BMC),bone metabolic index,and the expression of BMP-2,Smad4,Wnt-1,andβ-catenin were measured.ResultsAfter 12 weeks of treatment,the result of micro-CT and dual energy X-ray showed that the trabecular microscopic parameters,BMD,and BMC in XGS group were significantly improved compared to those in OVX group.BMD,BMC,TV/BV,Tb.N,Tb.Th,and Tb.Sp in XGS group were significantly better than those in OVX group.The result of serum test and biomechanical test showed that the maximum load and elastic modulus in XGS group were higher than those in OVX group(P<0.05).The levels of serum ALP,OPD,and OCN in XGS group were significantly lower than those in OVX group.Western blotting detection showed that the expression levels of BMP-2,Smad4,Wnt-1,andβ-catenin in XGS group were significantly higher than those in OVX group.Conclusion XGS mediates the protective effect on bone mass loss in ovariectomized rats through BMP-2/Smad4/Wnt/β-catenin signal pathway.

关 键 词：熊果酸 绝经后骨质疏松症 骨代谢指标 骨密度 BMP-2/Smad4/Wnt/β-catenin信号通路 

分 类 号：R-332[医药卫生]