双向电泳分离水稻叶片蛋白实验教学的探究  

Study on the Experiment Teaching of Separating Leaf Protein in Rice by Two-dimensional Electrophoresis

作　　者：王琳[1] 杨晓勤[2] WANG Lin;YANG Xiao-qin(College of Bioscience and Biotechnology,Yangzhou University,Yangzhou,Jiangsu 225009,China)

机构地区：[1]扬州大学生物科学与技术学院生化与分子生物学教研室,江苏扬州225009 [2]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《教育教学论坛》2020年第50期389-390,共2页Education And Teaching Forum

摘　　要：采用双向电泳法分离水稻叶片蛋白。主要比较和分析了两种不同pH胶条对实验的影响。改进的pH胶条灵敏度高,分离条带多且清晰。结果表明:改进的实验方法可以得到更为灵敏、清晰的电泳图谱,更好地适应和促进实验教学工作。Objective:To improve the method of separating leaf protein in rice by two-dimensional electrophoresis(2-DE).Methods:Leaf protein were separated by 2-DE.The two different methods were used respectively.The effects of two different pH tapes on experiments are analyzed by comparison.The improved pH tape has higher sensitivity,showing more separated strips and clearer.Results:The improved experiment method can obtain more sensitive and clear electrophoretic map,and better adapt and promote the experiment teaching work.

关 键 词：实验教学 双向电泳 叶片 

分 类 号：G642.0[文化科学—高等教育学]