UPLC-ESI-MS/MS法同时测定强力定眩胶囊中的7个指标成分  

Simultaneous Determination of Seven Index Compositions from Qiangli Dingxuan Capsules by UPLC-ESI-MS/MS

作　　者：王媚[1] 边敏琦 邹俊波 崔春利[1] 张小飞[1] 史亚军[1] 郭东艳[1] 乔安平 贾小刚 WANG Mei;BIAN Minqi;ZOU Junbo;CUI Chunli;ZHANG Xiaofei;SHI Yajun;GUO Dongyan;QIAO Anping;JIA Xiaogang(Shaanxi Key Laboratory of New Drugs and Chinese Medicine Foundation Research,College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西省中药基础与新药研究重点实验室,陕西中医药大学药学院,陕西咸阳712046

出　　处：《化学与生物工程》2020年第12期63-68,共6页Chemistry & Bioengineering

基　　金：陕西省科技厅项目(2017SF-308);陕西省中药制药重点学科资助项目(303061107)。

摘　　要：建立了同时测定强力定眩胶囊中天麻素、绿原酸、阿魏酸、蒙花苷、对羟基苯甲醇、松脂醇单葡萄糖苷、松脂醇二葡萄糖苷的超高效液相色谱-电喷雾-三重四级杆质谱法(UPLC-ESI-MS/MS)。采用Thermo Syncronis C18色谱柱(100 mm×2.1 mm,1.7μm),以0.1%甲酸水溶液-甲醇为流动相进行梯度洗脱,流速0.4 mL·min^-1,柱温35℃;采用电喷雾离子源(ESI)、多反应监测(MRM)模式进行定量分析。结果表明,天麻素、绿原酸、阿魏酸、蒙花苷、对羟基苯甲醇、松脂醇单葡萄糖苷、松脂醇二葡萄糖苷分别在0.0838~20.9673μg·mL^-1、0.2848~71.2174μg·mL^-1、0.0286~7.1615μg·mL^-1、0.2112~52.8701μg·mL^-1、0.0672~16.8124μg·mL^-1、0.0784~19.6124μg·mL^-1、0.0768~19.2342μg·mL^-1范围内与峰面积线性关系良好,相关系数R均大于0.9800,平均加标回收率在98.99%~101.92%之间。该方法简单、准确、灵敏度高,可用于强力定眩胶囊指标成分的测定。We established a UPLC-ESI-MS/MS method for the simultaneous determination of seven index compositions(gastrodin,chlorogenic acid,ferulic acid,linarin,hydroxybenzanol,pinoresinol singleglucoside,and pinoresinol diglucoside)from Qiangli Dingxuan Capsules.The analysis is performed on a Thermo Syncronis C18 column(100 mm×2.1 mm,1.7μm)with gradient elution of 0.1%formic acid aqueous solution-methanol,the flow rate is 0.4 mL·min^-1,and the column temperature is 35℃.The quantitative analysis is carried out by electrospray ion source(ESI)and multiple reaction monitoring(MRM)mode.The results show that there is a good linear relationship between following factor,such as gastrodin,chlorogenic acid,ferulic acid,linarin,hydroxybenzyl alcohol,pinoresinol singleglucoside,and pinoresinol diglucoside and the peak area in the range of 0.0838-20.9673μg·mL^-1,0.2848-71.2174μg·mL^-1,0.0286-7.1615μg·mL^-1,0.2112-52.8701μg·mL^-1,0.0672-16.8124μg·mL^-1,0.0784-19.6124μg·mL^-1,and 0.0768-19.2342μg·mL^-1,respectively.The correlation coefficients are greater than 0.9800,and the average adding standard recoveries are 98.99%-101.92%.The method is simple,accurate,and sensitive,and can be used for the determination of index compositions from Qiangli Dingxuan Capsules.

关 键 词：强力定眩胶囊 天麻素 绿原酸 阿魏酸 蒙花苷 对羟基苯甲醇 松脂醇单葡萄糖苷 松脂醇二葡萄糖苷 UPLC-ESI-MS/MS 

分 类 号：O657.63[理学—分析化学] R284.1[理学—化学]