辐射制备的水溶性CdS量子点对小鼠胚胎成骨前体细胞的毒理性研究  

Preparation of Water Soluble CdS Quantum Dots by Radiation Method and Its Cytotoxicity on Mouse Embryonic Osteoblast Precursor Cells in Vitro

作　　者：李婷婷 柳国 陈洪国 李月生 吴鸣虎 LI Ting-ting;CHEN Hong-guo;LI Yue-sheng(School of Pharmacy,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院药学院,湖北咸宁437100 [2]湖北科技学院核技术与化学生物学院

出　　处：《湖北科技学院学报（医学版）》2020年第6期467-471,F0002,共6页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北省科技厅(2018AFB150);咸宁市科技研究与开发项目(2019kj02);湖北科技学院科研创新团队项目(2020TD01,H2019005);湖北省大创项目(S201910927021)。

摘　　要：目的研究CdS量子点(QDs)的不同配体、粒径大小、辐射剂量、浓度等对小鼠胚胎成骨前体细胞(MC3T3-E1)细胞毒性的影响。方法在室温下,采用原位电子束辐射方法成功制备巯基乙酸(TGA)和巯基丙酸(MPA)修饰的CdS量子点。通过动态光散射和倒置荧光显微等对CdS量子点进行粒径和微区分布等表征以及MTT法检测细胞存活率。结果当TGA-CdS QDs的最佳辐射吸收剂量为10kGy时,荧光发射峰值的强度最高,其平均粒径为(5.5±0.2)nm,紫外可见吸收峰为404nm,荧光光谱的发射峰为550nm。MPA-CdS QDs的最佳辐射吸收剂量为20kGy时,其平均粒径为(9.4±0.2)nm,紫外吸收峰为360nm,荧光光谱发射峰为553nm。与TGA-CdS QDs共培养24 h后,细胞存活率与TGA-CdS QDs浓度呈负相关。随着TGA-CdS QDs浓度的增加,细胞存活率显著下降,由(97.49±2.63)%下降到(48.04±4.93)%。与TGA-CdS QDs共培养48h后,细胞毒理作用也呈浓度依赖性。当TGA-CdS QDs浓度为3.90625μg/mL时,细胞存活率较高,接近88.64%。当浓度提高到500μg/mL时,细胞存活率急剧下降到(41.66±3.32)%。将MC3T3-E1细胞与MPA-CdS QDs共培养24h后,随着MPA-CdS QDs浓度从3.90625μg/mL增加到500μg/mL,细胞存活率从(92.95±2.21)%下降到(70.78±3.42)%。48h时,在浓度62.5~125μg/mL的区域,细胞存活率明显下降,由(88.35±2.42)%下降到(53.89±2.81)%。结论水溶性CdS QDs对MC3T3-E1细胞具有一定的毒性和抑制活性。特别是与配体较长、尺寸较大的MPA-CdS量子点相比,配体较短、尺寸较小的TGA-CdS量子点对MC3T3-E1细胞的细胞毒性更显著。Objective The effects of different ligands,particle size,radiation dose and concentration of CdS quantum dots(QDs)on the cytotoxicity of osteogenic precursors(MC3T3-E1)in mouse embryos were studied.Methods CdS quantum dots were successfully prepared by in situ electron beam radiation at room temperature.The particle size and microdistribution of CdS quantum dots were characterized by dynamic light scattering and inverted fluorescence microscopy method.Cell viability was determined by MTT assay.Results When the optimal radiation absorption dose of TGA-CDS QDs was 10kGy,the fluorescence emission peak intensity was the highest,the average particle size was(5.5±0.2)nm,the UV-visible absorption peak was 404 nm,and the emission peak of fluorescence spectrum was 550 nm.MPA-CdS QDs optimal radiation absorption dose is 20 kGy,the average particle size of MPA-CDS QDs was(9.4±0.2)nm,the uv absorption peak was 360 nm,and the fluorescence spectral emission peak was 553 nm.The cell survival rate was negatively correlated with TGA-CdS QDs concentration after 24 h co-culture with the TGA-CdS QDs.With the increase of TGA-CdS QDs concentration,the cell survival rate decreased significantly from(97.49±2.63)%to(48.04±4.93)%.The cytotoxicity was also concentration dependent after 48 h co-culture with TGA-CdS QDs.When the TGA-CdS QDs concentration was 3.90625μg/mL,the cell survival rate was higher,close to 88.64%.As the concentration risesμg/mL 500 Time,Cell survival rate dropped sharply to(41.66±3.32)%.MC3T3-E1 cells were cultured with MPA-CdS QDs for 24 hours,With the increase of MPA-CdS QDs concentrationμg/mL 3.90625 to 500μg/mL,The cell survival rate decreased from(92.95±2.21)%to(70.78±3.42)%.48h,At concentrations 62.5~125μg/mL,Cell viability was significantly reduced,decreased from(88.35±2.42)%to(53.89±2.81)%respectively.Conclusion Water soluble CdS QDs has certain toxicity and inhibitory activity on MC3T3-E1 cells.Specifically,compared with MPA-CdS QDs with longer ligands and larger size,TGA-CdS QDs with shorter li

关 键 词：电子束辐射 量子点 细胞毒性 MC3T3-E1细胞 体外 

分 类 号：R-332[医药卫生]