薇甘菊叶的化学成分及抑菌活性研究  

Study on chemical composition and antibacterial activity of the leaves of Mikania micrantha H.B.K.

作　　者：马秋 王照国 杨雪[1,2] 李[1,2] MA Qiu;WANG Zhao-guo;YANG Xue;LI Yan(State Key Laboratory of Functions and Applications of Medicinal Plants;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs,Guiyang 550014,China)

机构地区：[1]贵州医科大学省部共建药用植物功效与利用国家重点实验室 [2]贵州省中国科学院天然产物化学重点实验室/贵州省天然药物工程研究中心,贵阳550014

出　　处：《天然产物研究与开发》2020年第12期2061-2065,共5页Natural Product Research and Development

基　　金：国家自然科学基金地区基金(31660530);国家重点研发计划子课题(2017YFD0201402-05);贵州省科学基金(黔科合支撑[2018]2357)。

摘　　要：薇甘菊(Mikania micrantha H.B.K)是一种入侵植物,已对入侵地的健康生态环境造成不利影响;本文采用硅胶柱色谱分离纯化方法,研究薇甘菊叶的化学成分,分离得到9个化合物,分别为十三烷酸甲酯(1)、(Z)-6-十九碳烯酸(2)、植酸棕榈酸酯(3)、十六烷酸(4)、α-生育酚醌(5)、黑麦草内酯(6)、豆甾醇(7)、薇甘菊内酯(8)和二氢薇甘菊内酯(9),其中1~6均为首次从薇甘菊中分离得到。采用肉汤二倍稀释法对化合物1~9进行抑菌活性测试,结果显示化合物8和9对所测受试菌都有抑菌活性,最小抑菌浓度均为500 mg/L。Mikania micrantha H.B.K is the most invasive plants in the world.The damage has severely affected the economic development of the invaded area;Nine compounds were isolated from the leaves of M.micrantha plants method through silica gel column chromatography,which were methyl tridecanoate(1),(Z)-6-nonadecenoic acid(2),phytyl palmitate(3),palmitic acid(4),α-tocopherolquinone(5),loliolide(6),stigmasterol(7),mikanolide(8),and dihydromikanolide(9)were isolated from leaves of M.micrantha by chromatography method.Their structures were elucidated on the basis of spectroscopic evidence.Compounds 1-6 were isolated from M.micrantha for the first time.The doubling dilution method of broth was used to test the antibacterial activity of compounds 1-9.The results showed that compounds 8 and 9 had antibacterial activity against the tested bacteria,and the minimum inhibitory concentration was 500 mg/L.

关 键 词：薇甘菊 抑菌活性 化学成分 

分 类 号：R284.2[医药卫生—中药学]