树莓果肉多糖超声提取工艺优化及其抗疲劳作用  

Ultrasonic extraction optimization of raspberry ( Rubus idaeus L.)pulp polysaccharides and its anti-fatigue activity

作　　者：吴云 陈薇 田文慧 杨永晶 WU Yun;CHEN Wei;TIAN Wen-hui;YANG Yong-jing(Qinghai University,College of Ecology-Environment Engineering;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学生态环境工程学院 [2]青海大学省部共建三江源生态与高原农牧业国家重点实验室,西宁810016

出　　处：《天然产物研究与开发》2020年第12期2081-2093,共13页Natural Product Research and Development

基　　金：青海省科技厅基础研究计划(2019-ZJ-953Q);国家自然科学基金(81860619)。

摘　　要：本研究利用响应面法对树莓果肉多糖的超声提取工艺进行优化,并通过游泳小鼠实验测评其抗疲劳活性。结果表明,树莓果肉多糖的最佳提取条件为温度58℃,时间84 min,超声功率58 W,料液比1∶20,此时多糖的提取得率高达11.75%。树莓果肉多糖在红外光谱中呈现出典型的多糖吸收峰;主要含有的单糖类型有半乳糖、鼠李糖、葡萄糖、木糖、甘露糖以及阿拉伯糖,且摩尔比分别为2.3∶2.0∶7.1∶1.0∶1.1∶19.9。通过游泳小鼠实验发现,树莓果肉多糖能有效提高游泳小鼠的运动耐力和运动适应性,维持血糖及糖原水平,减少乳酸产生,加速其清除,显示出较好的抗疲劳活性。In this study,the ultrasonic extraction process of raspberry pulp polysaccharides(RPP)was optimized using response surface methodology and the anti-fatigue activity of RPP was evaluated by swimming mice experiments.The results showed that the optimal ultrasonic extraction conditions of RPP were temperature 58℃,extraction time 84 min,ultrasonic power 58 W and material-liquid ratio 1∶20,in which the extract yield of RPP could reach 11.75%.RPP showed a typical polysaccharides infrared spectrum and was composed of galactose,rhamnose,glucose,xylose,mannose and arabinose,with the molar ratio of 2.3∶2.0∶7.1∶1.0∶1.1∶19.9.Furthermore,RPP exhibited significant anti-fatigue activity in the swimming mice experiments,for it could effectively improve the exercise endurance and adaptability of swimming mice,maintain the level of blood sugar and glycogen,reduce the production of lactic acid and accelerate its clearance.

关 键 词：树莓果肉多糖 超声提取 提取工艺优化 抗疲劳活性 小鼠游泳实验 

分 类 号：TS201[轻工技术与工程—食品科学]