超声波协同酶法提取黄山贡菊总黄酮及其抗氧化活性  

Ultrasonic Assisted Enzymatic Extraction of HGTF and Antioxidant Activity

作　　者：史建俊[1] 李伟伟[1] 王免 李秀秀 庄雪燕 SHI Jian-jun;LI Wei-wei;WANG Mian(School of Chemistry and Chemical Engineering,Huangshan University,Huangshan,Anhui 245041)

机构地区：[1]黄山学院化学化工学院,安徽黄山245041

出　　处：《安徽农业科学》2020年第24期174-177,180,共5页Journal of Anhui Agricultural Sciences

基　　金：安徽省教育厅自然科学项目(KJHS2016B07,KJHS2017B08);黄山学院启动项目(2018xkjq018);国家级大学生创新创业项目(201810375040,201810375010)。

摘　　要：[目的]黄山贡菊总黄酮(total flavonoids from Huangshan Gongju,HGTF)提取工艺优化和抗氧化活性测定。[方法]以HGTF提取率为指标,采用单因素和正交试验优化超声波协同酶法提取HGTF工艺。通过自由基清除试验评价HGTF的抗氧化活性。[结果]HGTF的最佳提取条件为酶解时间20 min、复合酶质量分数0.1%、纤维素酶和果胶酶配比1∶1、乙醇浓度60%、料液比1∶20(g∶mL)、超声时间40 min、超声功率280 W、超声温度50℃,HGTF提取率为2.78%。抗氧化活性试验结果表明,HGTF溶液对DPPH·、·OH和O 2·-的清除率较高,分别为90.47%(0.05 mg/mL)、73.44%(0.10 mg/mL)和78.73%(0.10 mg/mL)。[结论]复合酶解后超声波提取HGTF条件温和、提取率高。该研究为HGTF的应用提供参考依据。[Objective]To optimize the extraction process and antioxidant activity of HGTF.[Method]Taking the HGTF yield as index,the extraction process was optimized by single factor experiment and orthogonal experiment.The oxidation resistance of HGTF was evaluated by scavenging tests for different free radicals.[Result]The optimum extraction conditions were finally determined as follows,enzymolysis time of 20 min,mass fraction of complex enzyme of 0.1%,cellulase-pectinase ratio of 1∶1,ethanol concentration of 60%,solid-liquid ratio of 1∶20,ultrasonic time of 40 min,ultrasonic power of 280 W,ultrasonic temperature of 50℃,the extraction rate of HGTF was 2.78%.The results of antioxidant activity test showed that the scavenging rate of HGTF solution to DPPH·,·OH and O 2·-was high.The clearance rates to DPPH·,·OH and O 2·-were 90.94%(0.05 mg/mL),73.44%(0.10 mg/mL)and 78.73%(0.10 mg/mL),respectively.[Conclusion]Ultrasonic extraction of HGTF after complex enzymatic hydrolysis has mild conditions and high extraction rate.This research provides some reference for the further application of HGTF.

关 键 词：黄山贡菊 总黄酮 超声波协同酶法 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]