miR-32-5p低表达通过调控PI3K/AKT信号通路抑制胃癌细胞增殖、迁移、侵袭并诱导凋亡  

THE LOW EXPRESSION OF MIR-32-5P INHIBITS THE PROLIFERATION,MIGRATION,INVASION AND INDUCES APOPTOSIS OF GASTRIC CANCER CELLS BY REGULATING THE PI3K/AKT SIGNALING PATHWAY

作　　者：衣兰娟 衣兰杰[2] 任珺[1] 赵娜 丁艳菲 Yi Lanjuan;Yi Lanjie;Ren Jun(Department of Gastroenterology,Yantai Mountain Hospital,Yantai 264000,China)

机构地区：[1]山东省烟台市烟台山医院消化内科,264000 [2]南京中医药大学临床文献研究室

出　　处：《中国煤炭工业医学杂志》2020年第6期567-572,共6页Chinese Journal of Coal Industry Medicine

基　　金：山东省医药卫生科技发展计划项目(编号:2018WS035);烟台市科技计划项目(编号:2020YD033)。

摘　　要：目的探讨miR-32-5p调控PI3K/AKT信号通路对胃癌细胞增殖、迁移、侵袭和凋亡的影响。方法采用qRT-PCR检测人正常胃粘膜细胞GES-1和3种胃癌细胞(AGS、MKN-28和SGC-7901)中miR-32-5p的相对表达水平。以SGC-7901细胞为研究对象,构建抑制miR-32-5p表达的胃癌细胞株。采用MTT法检测细胞增殖情况,流式细胞术检测细胞凋亡情况,Transwell实验检测细胞的迁移和侵袭能力,Western blot检测增殖相关蛋白CyclinD1、凋亡相关蛋白Cleave-capase-3、迁移侵袭相关蛋白MMP2和MMP9以及PI3K/AKT信号通路下游蛋白p-PI3K和p-AKT表达水平。结果与人正常胃黏膜细胞GES-1比较,3种胃癌细胞中miR-32-5p的表达水平显著上调(均P<0.05)。抑制miR-32-5p表达可抑制CyclinD1、MMP2和MMP9蛋白表达,促进Cleave-capase-3蛋白表达,抑制胃癌细胞的增殖、迁移和侵袭,诱导细胞凋亡(均P<0.05)。抑制miR-32-5p表达能够抑制p-PI3K和p-AKT表达,抑制PI3K/AKT信号通路活化(均P<0.05)。激活PI3K/AKT信号通路能够逆转抑制miR-32-5p表达对胃癌细胞增殖、迁移、侵袭和凋亡的影响。结论 miR-32-5p低表达通过抑制PI3K/AKT信号通路进而抑制胃癌细胞增殖、迁移和侵袭,并诱导细胞凋亡。Objective To investigate the effect of miR-32-5 p on proliferation,migration,invasion and apoptosis of gastric cancer cells by regulating PI3 K/AKT signaling pathway.Methods The relative expression levels of miR-32-5 p in human gastric mucosal cells GES-1 and three gastric cancer cells(AGS,MKN-28 and SGC-7901) were detected by qRT-PCR.SGC-7901 cells were selected for the subsequently study,and the gastric cancer cell lines that with the inhibition of miR-32-5 p was constructed.Cell proliferation was detected by MTT method,apoptosis was detected by flow cytometry,and the ability of cell migration and invasion were detected by transwell assay.The expression level of proliferation-related proteins CyclinD1,apoptosis-related proteins Cleave-capase-3,migration-invasively related proteins MMP2 and MMP9,and downstream proteins of the PI3 K/AKT signaling pathway p-PI3 K and p-AKT were detected by Western blot.Results Compared with human normal gastric mucosal cells GES-1,the expression levels of miR-32-5 p were significantly up-regulated in the three gastric cancer cells(P<0.05).Inhibition of miR-32-5 p inhibited the expression of CyclinD1,MMP2 and MMP9 proteins,promoted the expression of Cleave-capase-3 protein,inhibited the proliferation,migration and invasion of gastric cancer cells,and induced apoptosis(P<0.05).Inhibition of miR-32-5 p inhibited p-PI3 K and p-AKT expression,thereby inhibited the activation of PI3 K/AKT signaling pathway(P<0.05).Activation of PI3 K/AKT signaling pathway could reverse the effect of inhibiting miR-32-5 p on proliferation,migration,invasion and apoptosis of gastric cancer cells.Conclusion The low expression of miR-32-5 p inhibits the proliferation,migration and invasion of gastric cancer cells and induces apoptosis by inhibiting the PI3 K/AKT signaling pathway.

关 键 词：miR-32-5p 胃癌 PI3K/AKT信号通路 增殖 迁移 侵袭 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]