长链非编码RNA SNHG16在结直肠癌癌组织中的表达及其对SW480细胞株增殖的影响  

EXPRESSION OF LONG NON-CODING RNA SNHG16 IN COLORECTAL CANCER TISSUES AND ITS EFFECTS ON PROLIFERATION OF SW480 CELL LINE

作　　者：韩帅 马俊霞 黄加国 Han Shuai;Ma Jun xia;Huang Jiaguo(Department of Gastroenterology,Jinan People's Hospital,Jinan 271100,China)

机构地区：[1]山东省济南市人民医院消化内科,271100

出　　处：《中国煤炭工业医学杂志》2020年第6期601-605,共5页Chinese Journal of Coal Industry Medicine

基　　金：山东省医药卫生科技发展计划资助项目(编号:2018WS478)。

摘　　要：目的研究长链非编码RNA(Lnc RNA)SNHG16在结直肠癌(CRC)癌组织中的表达及其对SW480细胞株增殖的影响。方法收集于该院接受肛肠科手术切除的38例CRC患者,分别检测患者癌组织和癌旁组织中lnc RNA SNHG16表达情况;体外培养结直肠癌SW480细胞,并将结直肠癌SW480细胞分为对照组和干扰组;干扰组使用siRNA敲低SNHG16的表达,使用RT-PCR检测二组SNHG16表达情况;采用克隆形成实验检测二组细胞的增殖情况。采用蛋白质印迹法检测二组增殖相关蛋白Ki-67、PCNA表达情况;采用流式细胞仪检测二组细胞周期情况。结果与癌组织比较,癌旁组织中SNHG16表达高(P <0.05);与对照组比较,干扰组SNHG16mRNA表达、Ki67蛋白相对表达、PCNA蛋白相对表达均较低(均P<0.05)。干扰组克隆形成率为(7.36±4.55)%,明显低于对照组(49.36±8.21)%(P <0.05)。干扰组SW480细胞G0/G1期细胞比例为(62.21±1.70)%,明显高于对照组(53.16±1.68)%;干扰组SW480细胞G2/M期和S期细胞比例为(15.80±0.50)%和(21.99±1.08)%,低于对照组(18.35±0.88)%和(28.49±1.25)%(P<0.05)。结论结直肠癌组织中长链非编码RNA SNHG16呈高表达,敲低SNHG16的表达可以通过降低增殖相关蛋白的表达并阻滞SW480细胞于G0/G1期,实现抑制SW480细胞的恶性增殖。Objective To study the expression of long non-coding RNA(lnc RNA) SNHG16 in colorectal cancer(CRC) tissues and its effects on proliferation of SW480 cell line.Methods Thirty eight CRC patients who underwent surgical resection in anorectal department of Jinan People′s Hospital were collected.The expression of lnc RNA SNHG16 in cancer tissues and para-cancerous tissues was detected.CRC SW480 cells were cultured in vitro.And they were divided into control groups and interference group.SNHG16 expression was knocked down by siRNA in interference group.SNHG16 expression in both groups was detected by RT-PCR.The cells proliferation in both groups was detected by clone formation assay.The expression of proliferation-related proteins Ki-67,PCNA in both groups was detected by Western blot.The cell cycles in both groups were detected by flow cytometry.Results Compared with cancer tissues,expression of SNHG16 in para-cancerous tissues was higher(P<0.05).Compared with control group,expression of SNHG16 mRNA,relative expression of Ki67 and PCNA proteins were lower in interference group(P<0.05).The clone formation rate in interference group was lower than that in control group[(7.36±4.55)% vs.(49.36±8.21)%](P<0.05).The proportion of SW480 cells in G0/G1 phase in interference group was higher than that in control group [(62.21±1.70)% vs.(53.16±1.68)%],while proportions of SW480 cells in G2/M phase and S phase were lower than those in control group [(15.80±0.50)%,(21.99±1.08)% vs.(18.35±0.88)%,(28.49±1.25)%](P<0.05).Conclusion The lnc RNA SNHG16 is highly expressed in CRC tissues.Knocking down SNHG16 expression can inhibit the malignant proliferation of SW480 cells by reducing the expression of proliferation-related proteins and blocking SW480 cells in G0/G1 phase.

关 键 词：长链非编码RNA SNHG16 结直肠癌 SW480细胞株 细胞增殖 

分 类 号：R735.3[医药卫生—肿瘤]