匹立尼酸对肝癌TAE术后癌旁肝组织过氧化物酶体增殖物激活受体-α、核因子-κB和基质金属蛋白酶-9表达的影响  

The effect of pirinixic acid on the expressions of PPAR-α,NF-κb and MMP-9 in paracancerous liver tissues after transarterial embolization of liver cancer

作　　者：范馨予 杜伟 李正亮 吴春华 杨培鈺 熊文翠 FAN Xinyu;DU Wei;LI Zhengliang;WU Chunhua;YANG Peiyu;XIONG Wencui(School of Clinical Medicine of Dali University,Department of Radiology,First Affiliated Hospital of Dali University,Dali,Yunnan Province 671000,China)

机构地区：[1]大理大学临床医学院,云南671000 [2]大理大学第一附属医院放射科

出　　处：《介入放射学杂志》2020年第11期1110-1115,共6页Journal of Interventional Radiology

基　　金：国家自然科学基金地区项目(81660300)。

摘　　要：目的探讨匹立尼酸对肝癌经导管动脉栓塞术(TAE)术后癌旁肝组织中过氧化物酶体增殖物激活受体(PPAR)-α、核因子(NF)-κB和基质金属蛋白酶(MMP)-9表达的影响。方法构建VX2肝癌兔模型并追踪肿瘤种植和生长情况。造模成功后将60只模型兔随机分为3组(每组20只),对照组不作任何处理,TAE组仅行TAE术,联合治疗组在TAE术前3 d连续注射匹立尼酸,随后行TAE术。化学比色法检测术前术后模型兔外周血中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平。荧光定量聚合酶链反应检测癌旁肝组织中PPAR-α、NF-κB和MMP-9 mRNA表达。免疫组化法检测术后癌旁肝组织蜡块中PPAR-α、NF-κB和MMP-9蛋白表达。结果三组模型兔间术前ALT、AST水平差异无统计学意义;联合治疗组术后ALT、AST水平高于对照组,低于TAE组,差异均有显著统计学意义。联合治疗组癌旁组织中PPAR-αmRNA表达显著高于对照组、TAE组,差异均有统计学意义;NF-κB mRNA、MMP-9 mRNA表达显著低于TAE组,差异均有统计学意义,但与对照组比较,差异均无统计学意义。联合治疗组癌旁组织中PPAR-α阳性率(70%)显著高于对照组(30%)、TAE组(20%);NF-κB(30%)、MMP-9(35%)阳性率低于TAE组(65%,75%),但与对照组(20%,25%)差异均无统计学意义。结论匹立尼酸可缓解肝癌TAE术后癌旁组织炎性反应和肝功能损伤,这可能与其促进PPAR-α表达、抑制NF-κB信号通路及其下游MMP-9表达有关。Objective To investigate the effects of pirinixic acid(WY14643)on the expressions of peroxisome proliferators-activated receptor-α(PPAR-α),nuclear factor-κb(NF-κb)and matrix metalloprotein-9(MMP-9)in paracancerous liver tissues after transarterial embolization(TAE)of liver cancer.Methods The rabbit VX2 liver cancer model was established,the implantation and growth of tumor were followed up.A total of 60 successful modeling rabbits were randomly and equally divided into 3 groups with 20 rabbits in each group.The rabbits of control group received no treatment,and the rabbits of TAE group received TAE only.For the rabbits of combination treatment group,continuous injection of WY14643 was adopted for three days which was followed by TAE.The serum levels of ALT and AST in peripheral blood of experimental rabbits were determined by chemical colorimetry before and after treatment.The mRNA expressions of PPAR-α,NF-κb and MMP-9 in paracancerous liver tissues were tested by fluorescence quantitative polymerase chain reaction.Immunohistochemistry was used to detect the protein expressions of PPAR-α,NF-κb and MMP-9 in paracancerous liver tissues.Results There were no statistically significant differences in preope rative ALT and AST levels between each other among the three groups.The postoperative levels of ALT and AST in the combination treatment group were higher than those in the control group,while lower than those in the TAE group,the differences were statistically significant.The expression of PPAR-αmRNA in the paracancerous liver tissues in the combination treatment group was significantly higher than that in the TAE group and in the control group,the difference was statistically significant.The expression levels of NF-κb mRNA and MMP-9 mRNA in the paracancerous liver tissues in the combination treatment group were significantly lower than those in the TAE group,the differences were statistically significant,but when compared with those in the control group the differences were not statistically significant

关 键 词：肝癌 匹立尼酸 过氧化物酶体增殖物激活受体-Α 核因子-ΚB 基质金属蛋白酶-9 

分 类 号：R735.7[医药卫生—肿瘤]