辣椒素对人子宫内膜癌细胞增殖凋亡影响的研究  

Effect of capsaicin on proliferation and apoptosis of human endometrial cancer cells

作　　者：马毓梅[1] 宋立党 左宏玲[2] 曹巧静[2] 刘宏[3] 何小静[4] 李晓冬[2] 郭燕红 MA Yumei;SONG Lidang;ZUO Hongling;CAO Qiaojing;LIU Hong;HE Xiaojing;LI Xiaodong;GUO Yanhong(Department of Pathology,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院病理科,石家庄050000 [2]石家庄河北医科大学第二医院妇科,050000 [3]河北医科大学第二医院体检中心,石家庄050000 [4]河北医科大学第二医院妇七科,石家庄050000 [5]沧州市人民医院生殖医学科 [6]河北省医科大学第三医院生殖医学

出　　处：《中国妇产科临床杂志》2020年第6期613-615,共3页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：河北省卫生计生委医学科学研究重点课题计划(20170537);河北省科技厅计划项目(172777760)。

摘　　要：目的探究辣椒素对人子宫内膜癌ISHIKAWA细胞增殖、凋亡的影响,并探讨其影响与辣椒素受体(TRPV1)的关系。方法采用细胞培养、MTT法、Hoechst 33342染色和流式细胞术观察不同浓度辣椒素对人子宫内膜癌ISHIKAWA细胞的增殖、凋亡和细胞周期的影响,Western Blot法观察凋亡相关蛋白(cleaved caspase-3)和周期相关蛋白(CDK2)表达的变化,以及辣椒素受体拮抗剂(辣椒平)干预后变化。结果不同浓度辣椒素处理后,ISHIKAWA细胞数量减少、皱缩程度加深、贴壁性降低和碎裂细胞增多;随辣椒素浓度增高,ISHIKAWA细胞增殖率降低、凋亡率升高,细胞周期阻滞于S期,cleaved caspase-3表达水平明显上调,CDK2表达水平明显下调。辣椒平参与处理后,上述变化均出现逆转。结论辣椒素可通过诱导细胞凋亡及S期阻滞抑制ISHIKAWA细胞的增殖,这种作用为TRPV1依赖性的,且可能与cleaved caspase-3和CDK2的表达异常相关。Objective To observe the influence of capsaicin on the proliferation and apoptosis of human endometrial carcinoma ISHIKAWA cells, and explore the relationship between the effect and capsaicin receptor(TRPV1). Methods Cell culture, MTT, Hoechst 33342 staining and flow cytometry were used to observe the effects of different concentrations of capsaicin on the proliferation, apoptosis and cell cycle of ISHIKAWA cells.The expression of apoptosis associated protein(cleaved caspase-3) and cyclin-related protein(CDK2) were detected by Western Blot analysis. And whether the antagonist of capsaicin receptor(capsazepine) interferes with the above effects and changes or not. Results After treatment with different concentration of capsaicin, the morphology of ISHIKAWA cells showed that the number of cells and the attachment of cells were both decreased, the cell shrinkage degree was aggravated, and the number of fragmented cells were increased. As the concentration of capsaicin increased, the proportion of ISHIKAWA cells proliferated decreased, the ratio of apoptosis was increased, and the cell cycle arrest occurred in the S-phase. The expression level of cleaved caspase-3 was increased markedly. The expression of CDK2 was significantly decreased. However, the above changes were reversed by capsazepine. Conclusion Capsaicin can inhibit the proliferation of ISHIKAWA cells by inducing apoptosis and S-phase block, which is TRPV1-dependent and may be related to abnormal expression of cleaved caspase-3 and CDK2.

关 键 词：子宫内膜癌 ISHIKAWA细胞 辣椒素 辣椒平 辣椒素受体 细胞凋亡 细胞周期阻滞 

分 类 号：R285[医药卫生—中药学]