骨髓单个核细胞移植对溃疡性结肠炎模型小鼠miRNA-21和miRNA-155表达影响  

Effect of bone marrow mononuclear cell transplantation on miRNA-21 and miRNA-155 expression in mice with ulcerative colitis

作　　者：杨松[1] 姜威[1] 鲍秀琦[1] 姚潍 陈刚[1] 张赫 陈曦 布琼 杨舒贺 戚亚男 王伟群[2] 韩宇鹏[1] Yang Song;Jiang Wei;Bao Xiuqi;Yao Wei;Chen Gang;Zhang He;Chen Xi;Bu Qiong;Yang Shuhe;Qi Yanan;Wang Weiqun;Han Yupeng(Department of Gastroenterology,the First Affiliated Hospital of Jiamusi University,Jiamusi 154002,China;School of Basic Medicine,Jiamusi University,Jiamusi 154002,China;School of Clinical Medicine,Jiamusi University,Jiamusi 154002,China)

机构地区：[1]佳木斯大学附属第一医院消化科,佳木斯154002 [2]佳木斯大学基础医学院,佳木斯154002 [3]佳木斯大学临床医学院,佳木斯154002

出　　处：《中华医学杂志》2020年第44期3529-3533,共5页National Medical Journal of China

基　　金：黑龙江省卫生计生委科研课题(2018199);黑龙江省省属高等学校基本科研业务费优秀创新团队建设项目(2019-KYYWF-1334);黑龙江省省属高等学校基本科研项目(2019-KYYWF-1346)。

摘　　要：目的:探讨骨髓单个核细胞移植对溃疡性结肠炎(UC)模型小鼠微小RNA(miRNA)-21和miRNA-155表达影响。方法:健康清洁级6~8周龄KM小鼠数字随机分为移植组、模型组和正常对照组,每组15只。移植组和模型组用葡聚糖硫酸钠(DSS)方法造模24 h,移植组小鼠经尾静脉注入0.4 ml 4′,6-二脒基-2-苯基吲哚(DAPI)标记的P3-BM-MNCs细胞悬液(3.2×10^6个/ml);模型组及正常对照组大鼠注入0.4 ml磷酸盐缓冲液(PBS)。采用UC疾病活动指数(DAI)对小鼠的一般情况进行评分;采用HE染色观察小鼠结肠组织病理变化;采用实时定量PCR检测miRNA-21和miRNA-155信使RNA(mRNA)表达。结果:正常对照组、模型组和移植组的DAI评分分别为0(0,1)、3.1(2.8,3.3)和2.7(2.4,3.1),与正常对照组比较,模型组和移植组小鼠DAI评分较高(P<0.05);与模型组比较,移植组小鼠DAI评分较低(P<0.05)。正常对照组、模型组和移植组的组织损伤大体评分为0(0,1)、3(3,4)和1(1,2),组织损伤病理评分分别为0(0,1)、16(12,16)和6(6,8),与正常对照组比较,模型组和移植组小鼠组织损伤评分较高(P<0.05);与模型组比较,移植组小鼠组织损伤评分较低(P<0.05)。正常对照组、模型组和移植组的miRNA-21 mRNA表达水平分别为0.87±0.15、2.38±0.29和1.59±0.32,miRNA-155 mRNA表达水平分别为1.87±0.46、7.38±1.97和3.92±0.84,与正常对照组比较,模型组和移植组小鼠miRNA-21和miRNA-155 mRNA表达较高(P<0.01);与模型组比较,移植组小鼠miRNA-21和miRNA-155 mRNA表达较低(P<0.05)。结论:骨髓单个核细胞移植可改善UC模型小鼠组织病理学评分和DAI评分,其机制可能与下调miRNA-21和miRNA-155 mRNA表达相关。Objective To investigate the effect of bone marrow mononuclear cell transplantation on the expression of miRNA-21 and miRNA-155 in mice with ulcerative colitis(UC).Methods Healthy and clean KM mice aged 6-8 weeks were randomly divided into transplantation group,model group and normal control group with 15 mice in each group.In the transplantation group and model group,dextran sodium sulfate(DSS)was used to establish the model for 24 h.The mice in the transplantation group were injected with 0.4 ml of 4′,6-diaminol-2-phenylindole(DAPI)-labeled P3-BM-MNCs cell suspension(3.2×10^6 cells/ml),and the mice in the model group and the normal control group were injected with 0.4 ml phosphate buffer(PBS).UC disease activity index(DAI)was used to test the general condition of mice;HE staining was used to observe the pathological changes of colon tissue;Real-time quantitative PCR was used to detect the expression of miRNA-21 and miRNA-155 mRNA.Results DAI scores of normal control group,model group and transplantation group were 0(0,1),3.1(2.8,3.3)and 2.7(2.4,3.1),respectively.Compared with normal control group,the DAI score of model group and transplantation group was higher(P<0.05),and the DAI score of transplantation group was lower than that of model group(P<0.05).The gross scores of tissue injury in normal control group,model group and transplantation group were 0(0,1),3(3,4)and 1(1,2),respectively,and the pathological scores of tissue injury were 0(0,1),16(12,16)and 6(6,8),respectively,compared with the normal control group.The tissue injury score of the model group and the transplantation group was higher(P<0.05),and the tissue injury score of the transplantation group was lower than that of the model group(P<0.05).The expression levels of miRNA-21 mRNA in normal control group,model group and transplantation group were 0.87±0.15,2.38±0.29 and 1.59±0.32,respectively,and the expression levels of miRNA-155 mRNA were 1.87±0.46,7.38±1.97 and 3.92±0.84,respectively,compared with the normal control group,the expressi

关 键 词：单核细胞 溃疡性结肠炎 微小RNA-21 微小RNA-155 

分 类 号：R574.62[医药卫生—消化系统]