单侧前牙反[牙合]对三叉神经感觉主核背内侧部及丘脑腹后内侧核VGLUT表达水平影响的研究  

Effect of Unilateral Anterior Crossbite on VGLUT Expression in the Dorsomedial Part of the Trigeminal Nucleus and Ventral Posteromedial Thalamic Nucleus

作　　者：时铭鸿 刘鑫[2,3] 谢勉姣[2] 王冬梅 李金莲[4] 王美青[1,2] SHI Minghong;LIU Xin;XIE Mianjiao;WANG Dongmei;LI Jinlian;WANG Meiqing(Department of Stomatology,The Third Affiliated Hospital,Xinxiang Medical College,Xinxiang 453000,Henan Province;Department of Oral Anatomy and Physiology,School of Stomatology,The Fourth Military Medical University,Xi′an 710032,Shaanxi Province;Department of Stomatology,the 960th Hospital of People′s Liberation Army,Jinan 250031,Shandong Province;Department of Anatomy,Histology and Embryology,The Fourth Military Medical University,Xi′an 710032,Shaanxi Province,China)

机构地区：[1]新乡医学院第三附属医院口腔科,河南新乡453000 [2]第四军医大学口腔医学院口腔解剖生理学教研室,陕西西安710032 [3]中国人民解放军第960医院口腔科,山东济南250031 [4]第四军医大学人体解剖与组织胚胎学教研室,陕西西安710032

出　　处：《口腔颌面外科杂志》2020年第6期362-368,共7页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金项目(81870866)。

摘　　要：目的:异常咬合信息可以通过牙周本体感受器、三叉神经系向高级中枢传递,三叉神经感觉主核背内侧部(dorsomedial part of the trigeminal nucleus,Vpdm)-丘脑腹后内侧核(ventral posteromedial thalamic nucleus,VPM)是重要的三叉神经系上传通路,Vpdm是否会受到异常咬合影响,是否继续上传至VPM值得深入研究。本文通过检测单侧前牙反[牙合](unilateral anterior crossbite,UAC)大鼠,Vpdm及VPM中兴奋性神经传导标志物—囊泡膜谷氨酸转运体1,2(vesicular glutamate transporter1,2,VGLUT1,2)的表达变化,探讨该问题。方法:将雌性大鼠42只分为UAC组和对照组,造模后2、4和8周取材,以免疫荧光、Western blot及实时定量PCR技术,检测目标神经核团中VGLUT1,2的表达差异。结果:UAC组Vpdm内VGLUT1蛋白和mRNA的表达在各个时间点均明显高于对照组,而VGLUT2 mRNA的表达则无显著性差异。在VPM内,VGLUT1,2的蛋白水平和mRNA水平两组间均无显著差异。结论:UAC促进了Vpdm的VGLUT1表达,但对VPM的VGLUT1,2表达水平没有显著影响。Objective:Periodontal proprioceptional message derived from abnormal occlusion is transmitted via projections from mesencephalic trigeminal nucleus to dorsomedial part of the trigeminal nucleus(Vpdm)and then to ventral posteromedial thalamic nucleus(VPM).To elucidate whether abnormal occlusion will send impact on Vpdm and then on VPM,the expression levels of vesicular glutamate transporter1,2(VGLUT1,2),markers of excitatory nucleus,were detected in Vpdm and VPM of the unilateral anterior crossbite(UAC)treated rats.Methods:The female rats were divided into UAC and control group,and were killed at 2,4 and 8 week respectively.The expression levels of VGLUT1,2 were detected through immunofluorescence,Western blot and real-time PCR assays.Results:In UAC group,the VGLUT1 protein and mRNA expression levels in Vpdm were increased at all the three time points,while that of VGLUT2 showed no significant differences from the age-matched controls.No such a difference was noticed in the expression levels of VGLUT1,2 in VPM at both protein and mRNA levels.Conclusions:UAC promoted VGLUT1 expression in Vpdm,but showed no significant impact on the expression of VGLUT1,2 in VPM.

关 键 词：咬合 三叉神经感觉主核背内侧部 丘脑腹后内侧核 囊泡膜谷氨酸转运体 三叉神经传导通路 

分 类 号：R782[医药卫生—口腔医学]