滇黄精多糖的提取工艺及其抗氧化活性研究  

A study of the extraction and antioxidation activity of polysaccharide from Polygonatum kingianum

作　　者：李智敏[1] 石瑶[1] 赵纯希 于丽娟 张易莲 张庭[1] 李晚谊[1] 肖丹[1] LI Zhi-mi;SHI Yao;ZHAO Chun-xi;YU Li-juan;ZHANG Yi-lian;ZHANG Ting;LI Wan-yi;XIAO Dan(Institute of Medicinal Plants,Yunnan Academy of Agricultural Sciences,Kunming 650223,China;Institute of Agro-Products Processing,Yunnan Academy of Agricultural Sciences,Kunming 650221,China;College of Tropical Crop Science,Yunnan Agricultural University,Pu'er 665099,China)

机构地区：[1]云南省农业科学院药用植物研究所,云南昆明650200 [2]云南省农业科学院农产品加工研究所,云南昆明650223 [3]云南农业大学热带作物学院,云南普洱665099

出　　处：《云南民族大学学报（自然科学版）》2020年第6期535-540,共6页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：云南省重点研发计划(农业领域)(2018BB024);云南省重大科技专项(2017AB004)。

摘　　要：通过单因素和正交试验对多糖提取工艺中的料液比、提取时间、提取温度等进行优化,并以维生素C(Vc)为阳性对照,对滇黄精多糖进行抗氧化活性研究.结果表明,在最佳提取工艺即料液比为1∶20,提取时间为2 h,提取温度为80℃时,滇黄精多糖提取率达13.34%,并且在一定浓度下,滇黄精多糖对DPPH自由基、羟基自由基以及超氧阴离子自由基有不同程度清除作用,具有一定的抗氧化活性.In this research, the effects of the material-liquid ratio, the extraction time and extraction temperature in the extraction process of polysaccharide from Polygonatum kingianum were investigated by the single-factor and orthogonal tests. And the antioxidant activity of polysaccharide was studied by using vitamin C(Vc) as a positive control. The results showed that the best extraction conditions of polysaccharide from Polygonatum kingianum were as follows: the material-liquid ratio was 1∶20, the extraction time was 2 h, and the extraction temperature was 80 ℃. Under the best extraction conditions, the extraction rate of polysaccharide was 13.34%. And polysaccharide from Polygonatum kingianum showed some ability to scavenge DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals to varying degrees, and had certain antioxidant activity.

关 键 词：滇黄精多糖 提取工艺 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]