GC-MS法分析桑白皮中的脂溶性及挥发性成分  

Analysis on fat-soluble components and volatile components of Mori Cortex by GC-MS

作　　者：刘一涵[1] 郭洪伟 曹彤 李明 魏华[1,2] LIU Yihan;GUO Hongwei;CAO Tong;LI Ming;WEI Hua(College of Biology and Environmental Science,Jishou University,Jishou,Hunan,416000 P.R.China;Tujia Medicine Research Center in Hunan,Jishou,Hunan,416000 P.R.China)

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]湖南省土家医药研究中心,湖南吉首416000

出　　处：《华西药学杂志》2020年第6期644-649,共6页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81403088);中医药行业科研专项资助项目(201507002);湖南省土家医药研究中心开放项目(TJY201901);吉首大学校级科研项目资助(Jdy1858);吉首大学森林生态系统观测研究生培养创新基地资助(JSUFI201806)。

摘　　要：目的分析桑白皮中的脂溶性和挥发性成分。方法采用超声法提取脂溶性成分,并进行甲酯化处理,采用水蒸气蒸馏法提取挥发性成分,利用气质联用技术结合保留指数法分析鉴定脂溶性和挥发性成分。结果分别从脂溶性和挥发性成分中鉴定了39、52个化合物。脂溶性成分中含量较高的为:α-香树脂醇乙酸酯(39.40%)、β-香树脂醇乙酸酯(16.19%)、β-生育酚(7.00%)、乙酸羽扇醇酯(6.71%)、β-白檀酮(5.54%)、十六醛(4.09%),挥发性成分中含量较高的为D-(+)-纤维二糖八乙酸酯(51.82%)、亚油酸(11.81%)、亚麻酰氯(6.29%)。结论研究结果为进一步认识桑白皮的化学成分及其药效物质研究提供了依据。OBJECTIVE To analyze the fat-soluble components and volatile components in Mori Cortex.METHODS The fat-soluble components were extracted by ultrasonic method and methyl esterified,the volatile components were extracted by steam distillation method,and then identified them by gas chromatography-mass spectrometry technology and retention indices.RESULTS 39 and 52 constituents from the fat-soluble components and volatile components were identified.The constituents with higher content in fat-soluble components were:α-amyrin acetate(39.40%),β-amyrin acetate(16.19%),β-tocopherol(7.00%),lupenyl acetate(6.71%),β-amyrone(5.54%)and hexadecanal(4.09%).The constitutes with higher content in volatile components were D-ellobiose octaacetate(51.82%),linoleic acid(11.81%)and linoleoyl chloride(6.29%).CONCLUSION This study provides a theoretical basis for further understanding of the chemical constituents of Mori Cortex and its pharmacological research.

关 键 词：桑白皮 超声法 水蒸气蒸馏法 气质联用 保留指数 脂溶性成分 挥发性成分 甲酯化 

分 类 号：R917[医药卫生—药物分析学]