白血病抑制因子通过JAK1/STAT3信号通路调控非小细胞肺癌细胞活性  

Leukemia inhibitory factor regulating cell activity of non-small cell lung cancer through the JAK1/STAT3 signaling pathway

作　　者：黄科峰 江明君 王华英 戚赛春 HUANG Kefeng;JIANG Mingjun;WANG Huaying;QI Saichun(Department of Cardiothoracic Surgery,the Affiliated People’s Hospital Ningbo University School of Medicine,Ningbo 315040,China)

机构地区：[1]宁波大学附属人民医院心胸外科,浙江宁波315040

出　　处：《温州医科大学学报》2020年第12期970-973,共4页Journal of Wenzhou Medical University

基　　金：浙江省自然科学基金资助项目(2019A610229)。

摘　　要：目的:探讨白血病抑制因子(LIF)对非小细胞肺癌(NSCLC)的潜在作用及其机制。方法:采用免疫组织化学法、荧光定量PCR法、Western blot法检测LIF在NSCLC患者癌旁组织和肿瘤组织中的表达情况;采用CCK-8法和Transwell小室法检测LIF对肿瘤细胞A549细胞的增殖和侵袭作用的影响;Western blot检测JAK1/STAT3信号通路蛋白表达情况。结果:LIF在NSCLC的癌组织中的表达显著高于癌旁组织(P<0.01),经LIF共孵育后,肿瘤细胞的增殖和侵袭能力均明显增强(P<0.05);Western blot显示肿瘤细胞中JAK1、STAT3磷酸化水平明显提高(P<0.05)。结论:LIF在NSCLC中表达增加,可能通过JAK1/STAT3信号通路介导LIF的增加,并增强肿瘤细胞的增殖和侵袭能力。Objective:To investigate the effect of leukemia inhibitor factor(LIF)on non-small cell lung cancer(NSCLC)and its possible mechanism.Methods:Immunohistochemical,fluorescent quantitative PCR and Western blot were used to detect the expression of LIF in non-small cell lung cancer patients paracancer tissue and tumor tissue.The effect of LIF on the proliferation and invasion of tumor cell A549 cells was detected by CCK-8 and Transwell chamber.Western blot was used to detect the expression of JAK1/STAT3 signaling pathway protein.Results:The expression of LIF in cancer tissues of NSCLC was significantly higher than that of paracancer tissues(P<0.01).After co-incubation with LIF,the proliferation and invasion ability of tumor cells were significantly enhanced(P<0.05).Western blot showed that tumor cells JAK1 and STAT3 phosphorylation levels were significantly increased(P<0.05).Conclusion:LIF expression is increased in NSCLC.The increased LIF can enhance the proliferation and invasion ability of tumor cells,which may be mediated through the JAK1/STAT3 signaling pathway.

关 键 词：白血病抑制因子 STAT3 免疫组化 非小细胞肺癌 肿瘤细胞活性 

分 类 号：R734.2[医药卫生—肿瘤]