利用功能性分子标记辅助选育香型杂交水稻不育系  

Breeding of Fragrant Hybrid Rice Cytoplasmic Male Sterility(CMS)Line Using Molecular Marker-Assisted Selection

作　　者：王天生 陈惠清 谢旺有 陈锦文 谢少和 黄荣裕[2] Wang Tiansheng;Chen Huiqing;Xie Wangyou;Chen Jinwen;Xie Shaohe;Huang Rongyu(Quanzhou Institute of Agricultural Science,Quanzhou,362200;School of Life Sciences,Xiamen University,Xiamen,361102)

机构地区：[1]福建省泉州市农业科学研究所,泉州362200 [2]厦门大学生命科学学院,厦门361102

出　　处：《分子植物育种》2020年第23期7801-7807,共7页Molecular Plant Breeding

基　　金：水稻生物学国家重点实验室开放课题(170101);福建省种业创新与产业化工程2017-2020年(fjzycxny2017-004);福建省科技计划项目(2017N0201;2017N1005);福建省作物设计育种重点实验室2018年度开放项目(2018FJCBD01);泉州市科技项目(2018N051)共同资助。

摘　　要：本研究采用KOH浸泡法和咀嚼法分别对11个水稻品种的叶片及稻米进行香味鉴定,结果表明‘宜香1B’等9个品种表现为具有香味,‘祥B’、‘桐B’等两个品种表现为不具有香味。利用已克隆的水稻香味基因Badh2的序列信息,设计基于目的基因的功能性分子标记FGR3,进一步对上述品种的香味基因Badh2进行检测,结果表明,‘宜香1B’等9个表型鉴定为香型的水稻品种与表型鉴定为非香型的品种‘祥B’、‘桐B’在该位点表现出不同的分子标记基因型,表型与基因型鉴定结果相吻合。表明该标记可用于水稻香味的分子标记辅助选择。为选育出具有香味的优良水稻不育系,本研究以携带香味基因的保持系‘宜香1B’为香味基因供体材料,采用本单位育成的具有强配合力的保持系‘祥B’为受体材料,配制杂交组合并进行回交,对后代材料进行分子标记辅助选择,育成香型保持系株系,同时进行不育系转育,育成了优质香型不育系‘泉香1A’,目前正对其进一步进行配组利用研究。In order to identify the fragrance of rice varieties,leaf and grains from 11 rice varieties were detected in the study.Results showed that 9 of the 11 varieties were fragrant and 2 were non-fragrant.Based on the sequence information of cloned rice fragrant gene Badh2,a functional molecular marker FGR3 was developed and used to detect the above varieties on their fragrant genes.The results revealed that genotypes of the 9 fragrant varieties were different from the 2 non-fragrant varieties.The results of phenotype identification were consistent with their genotypes.So the functional marker FGR3 could be used on marker-assisted selection of fragrant rice.In order to develop new fine quality rice cytoplasmic male sterility(CMS)lines,a maintain line’XiangB’was used as recurrent parent and crossed with fragrant maintain line’Yixiang1 B’as donor parent.Backcrossing and marker-assisted selection was subsequently conducted to develop new fragrant maintain lines and CMS lines.After several years of selection,’Quanxiang1 A’,a CMS line,characteristic of fine quality and fragrance was developed.A series of hybrid combinations using’Quanxiang1 A’as female parent were on field testing.

关 键 词：功能性分子标记 辅助选育 香型 杂交水稻 不育系 

分 类 号：S511[农业科学—作物学]