以椰子织蛾为寄主的麦蛾柔茧蜂的冷藏研究  

Study on the cold storage of Habrobracon hebetor withOpisina arenosella as the host

作　　者：王雪梅 肖利锋[3] 吕宝乾 卢辉[1] 唐继洪[1] WANG Xuemei;XIAO Lifeng;Lü Baoqian;LU Hui;TANG Jihong(Institute of Environment and Plant Protection,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops,Ministry of Agriculture and Rural Areas/Hainan Provincial Key Laboratory of Biological Safety and Molecular Breeding,Haikou,Hainan 571101,China;College of Forestry,Hainan University,Danzhou,Hainan 571737,China;Dalian Join King Fine Chemical Co.,Ltd,Dalian,Liaoning 116600,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省南繁生物安全与分子育种重点实验室,海南海口571101 [2]海南大学林学院,海南儋州571737 [3]大连九信精细化工有限公司,辽宁大连116600

出　　处：《生物安全学报》2020年第4期268-272,共5页Journal of biosafety

基　　金：海南省自然科学基金创新研究团队项目(2019CXTD409);国家重点研发计划(2019YFD03001);农业部国际交流与合作项目(BARTP-08-LBQ);中国热带农业科学院基本科研业务费专项(1630042017011)。

摘　　要：【目的】探究麦蛾柔茧蜂最适的冷藏温度及虫态,为麦蛾柔茧蜂在生物防治的应用提供基础。【方法】将麦蛾柔茧蜂的4种虫态(卵、幼虫、蛹和成虫)置于-4、0、4、10℃温度下冷藏,观察其存活率,以确定其冷藏的最适虫态和最适温度。随后测定最适冷藏条件下,麦蛾柔茧蜂的生命参数。【结果】致死中时间(T 50)表明,麦蛾柔茧蜂以蛹期在10℃条件下冷藏为最佳,冷藏12 d时存活率仍达到75.25%。麦蛾柔茧蜂在10℃条件下冷藏后,首日产卵量在冷藏后期显著下降,麦蛾柔茧蜂性比显著降低,但冷藏3 d后其均值又逐渐恢复。茧蜂发育历期在冷藏后无显著变化。【结论】以椰子织蛾为寄主的麦蛾柔茧蜂于蛹期在10℃条件下冷藏12 d仍具有良好的利用价值,这可为麦蛾柔茧蜂在生物防治中的应用提供依据。【Aim】The study examined the potential of cold temperature for the storage of the biological control Habrobracon hebetor,so as to provide a basis for the application of H.hebetor in biological control.【Method】The four stages(egg,larva,pupa and adult)of the parasitoid H.hebetor were stored at-4,0,4,10℃and their survival rates were observed to determine the optimum cold storage stage and temperature.Then,the life parameters of the parasitoid were determined under the optimum cold storage conditions.【Results】The median lethal time(T 50)showed that the optimum cold storage condition for the parasitoid were pupa stage at 10℃.The survival rate was 75.25%after 12 days of cold storage.After cold storage at 10℃,the fecundity and sex ratio decreased significantly.While the average value gradually recovered after 3 days of cold storage.There was no significant difference in the developmental duration of the parasioid after cold storage.【Conclusion】The parasitoid could still be used after 12 days cold storage at 10℃in the pupal stage,which provided a basic information for the application of the parasitoid in biological control.

关 键 词：麦蛾柔茧蜂 椰子织蛾 冷藏 生物防治 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]