Identification of key genes involved in post-traumatic stress disorder:Evidence from bioinformatics analysis  

作　　者：Yao-Yao Bian Li-Li Yang Bin Zhang Wen Li Zheng-Jun Li Wen-Lin Li Li Zeng 

机构地区：[1]School of Nursing,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China [2]School of First Clinical Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China [3]Jingwen Library,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China [4]Digestive Department,Ningbo Hospital of Traditional Chinese Medicine,Ningbo 315200,Zhejiang Province,China [5]School of Preclinical Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou Province,China [6]Management School,University of St Andrews,St Andrews KY169AJ,United Kingdom [7]College of Health Economics Management,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China

出　　处：《World Journal of Psychiatry》2020年第12期286-298,共13页世界精神病学杂志

基　　金：Supported by the National Natural Science Foundation of China,No.81603529,81673982,and 81704084;the Natural Science Foundation of the Jiangsu Higher Education Institutions,No.16KJB360002;the Advantages of the Nursing Discipline Project of Jiangsu Province,No.2019YSHL005;China Scholarship Council,No.201908320373;the Jiangsu Government Scholarship for Overseas Studies;and the Qing Lan Project,No.014000773/2018-00376.

摘　　要：BACKGROUND Post-traumatic stress disorder(PTSD)is a serious stress-related disorder.AIM To identify the key genes and pathways to uncover the potential mechanisms of PTSD using bioinformatics methods.METHODS Gene expression profiles were obtained from the Gene Expression Omnibus database.The differentially expressed genes(DEGs)were identified by using GEO2R.Gene functional annotation and pathway enrichment were then conducted.The gene-pathway network was constructed with Cytoscape software.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis was applied for validation,and text mining by Coremine Medical was used to confirm the connections among genes and pathways.RESULTS We identified 973 DEGs including 358 upregulated genes and 615 downregulated genes in PTSD.A group of centrality hub genes and significantly enriched pathways(MAPK,Ras,and ErbB signaling pathways)were identified by using gene functional assignment and enrichment analyses.Six genes(KRAS,EGFR,NFKB1,FGF12,PRKCA,and RAF1)were selected to validate using qRT-PCR.The results of text mining further confirmed the correlation among hub genes and the enriched pathways.It indicated that these altered genes displayed functional roles in PTSD via these pathways,which might serve as key signatures in the pathogenesis of PTSD.CONCLUSION The current study identified a panel of candidate genes and important pathways,which might help us deepen our understanding of the underlying mechanism of PTSD at the molecular level.However,further studies are warranted to discover the critical regulatory mechanism of these genes via relevant pathways in PTSD.

关 键 词：Post-traumatic stress disorder Differentially expressed genes Key pathway Gene-pathway co-expression Bioinformatics analysis MICROARRAY 

分 类 号：R749.5[医药卫生—神经病学与精神病学] Q811.4[医药卫生—临床医学]