Lactobacillus bulgaricus inhibits colitis-associated cancer via a negative regulation of intestinal inflammation in azoxymethane/dextran sodium sulfate model  

作　　者：Denise Sayuri Calheiros Silveira Luciana Chain Veronez Luis Carlos Lopes-Junior Elen Anatriello Mariangela Ottoboni Brunaldi Gabriela Pereira-da-Silva 

机构地区：[1]Department of Biochemistry and Immunology,University of São Paulo at Ribeirão Preto Medical School,Ribeirão Preto 14049-900,SP,Brazil [2]Health Sciences Center,Federal University of Espírito Santo-UFES,Vitória 29043-900,ES,Brazil [3]Institute of Science and Technology,Federal University of São Paulo,UNIFESP,São Josédos Campos 12231-280,SP,Brazil [4]Department of Pathology and Forensic Medicine,University of São Paulo,Ribeirao Preto 14040-902,SP,Brazil [5]Department of Maternal-Infant Nursing and Public Health,University of São Paulo at Ribeirão Preto College of Nursing,Ribeirão Preto 14040-902,SP,Brazil

出　　处：《World Journal of Gastroenterology》2020年第43期6782-6794,共13页世界胃肠病学杂志（英文版）

基　　金：Supported by Brazilian National Council for Scientific and Technological Development(CNPq),No.140152/2013-0.

摘　　要：BACKGROUND Colitis-associated cancer(CAC)accounts for 2%-3%of colorectal cancer(CRC)cases preceded by inflammatory bowel diseases(IBD)such as Crohn's disease and ulcerative colitis.Intestinal microbiota has been reported to play a central role in the pathogenesis of IBD and CAC.Recently,numerous prebiotics and probiotics have being investigated as antitumor agents due to their capacity to modulate inflammatory responses.Previous studies have indicated that lactic acid bacteria could be successfully used in managing sporadic CRC,however little is known about their role in CAC.AIM To investigate the effect of the probiotic Lactobacillus bulgaricus(L.bulgaricus)during the development of an experimental model of colitis associated colon cancer(CAC).METHODS C57BL/6 mice received an intraperitoneal injection of azoxymethane(10 mg/kg),followed by three cycles of sodium dextran sulphate diluted in water(5%w/v).Probiotic group received daily L.bulgaricus.Intestinal inflammation was determined by scoring clinical signs.Cytokines levels were determined from colon and/or tumor samples by ELISA BD OptEIATM kits.The level of significance was set at P<0.05.Graphs were generated and statistical analysis performed using the software GraphPad Prism 6.0.RESULTS L.bulgaricus treatment inhibited of total tumor volume and mean size of tumors.In addition,the probiotic also attenuated the clinical signs of intestinal inflammation inducing a decrease in intestinal and tumor levels of IL-6,TNF-α,IL-17,IL-23 and IL-1β.CONCLUSION Our results suggest a potential chemopreventive effect of probiotic on CAC.L.bulgaricus regulates the inflammatory response and preventing CAC.

关 键 词：Lactobacillus bulgaricus Colitis-associated cancer Colorectal cancer CARCINOGENESIS Probiotics Inflammation 

分 类 号：R735.34[医药卫生—肿瘤]