Role of doublecortin-like kinase 1 and leucine-rich repeat-containing G-protein-coupled receptor 5 in patients with stage Ⅱ/Ⅲ colorectal cancer:Cancer progression and prognosis  

作　　者：Xue-Ling Kang Li-Rui He Yao-Li Chen Shu-Bin Wang 

机构地区：[1]Department of Oncology,Peking University Shenzhen Hospital,Shenzhen-Peking University-Hong Kong University of Science and Technology Medical Center,Shenzhen 518036,Guangdong Province,China [2]Department of Gastrointestinal Surgery,Peking University Shenzhen Hospital,Shenzhen 518036,Guangdong Province,China [3]Department of Pathology,Peking University Shenzhen Hospital,Shenzhen 518036,Guangdong Province,China [4]Department of Oncology,Peking University Shenzhen Hospital,Shenzhen Key Laboratory of Gastrointestinal Cancer Translational Research,China Cancer Institute of Shenzhen-Peking University-Hong Kong University of Science and Technology Medical Center,Shenzhen 518036,Guangdong Province,China

出　　处：《World Journal of Gastroenterology》2020年第43期6853-6866,共14页世界胃肠病学杂志（英文版）

基　　金：Supported by Sanming Project of Shenzhen,No.SZSM201612041;Shenzhen Science and Technology Innovation Commission Project,No.GJHZ20180420180754917 and No.ZDSYS20190902092855097;Postdoctoral Science Foundation of China,No.2018M633095.

摘　　要：BACKGROUND Cancer stem cells(CSCs)are a subpopulation of cancer cells with the potential of self-renewal and differentiation.CSCs play critical roles in tumorigenesis,recurrence,metastasis,radiation tolerance and chemoresistance.AIM To assess the expression patterns and clinical potential of doublecortin-like kinase 1(DCLK1)and leucine-rich repeat-containing G-protein-coupled receptor 5(Lgr5),as prognostic CSC markers of colorectal cancer(CRC).METHODS The expression of DCLK1 and Lgr5 in CRC tissue sections from 92 patients was determined by immunohistochemistry.Each case was evaluated using a combined scoring method based on signal intensity staining(scored 0-3)and the proportion of positively stained cancer cells(scored 0-3).The final staining score was calculated as the intensity score multiplied by the proportion score.Low expression of DCLK1 and Lgr5 was defined as a score of 0-3;high expression of DCLK1 and Lgr5 was defined as a score of≥4.Specimens were categorized as either high or low expression,and the correlation between the expression of DCLK1 or Lgr5 and clinicopathological factors was investigated.RESULTS DCLK1 and Lgr5 expression levels were significantly positively correlated.CRC patients with high DCLK1,Lgr5 and DCLK1/Lgr5 expressions had poorer progression-free survival and overall survival.Moreover,high expression of DCLK1 was an independent prognostic factor for recurrence and overall survival in patients with CRC by multivariate analysis(P=0.026 and P=0.049,respectively).CONCLUSION DCLK1 may be a potential CSC marker for the recurrence and survival of CRC patients.

关 键 词：Colorectal cancer Cancer stem cells Doublecortin-like kinase 1 Leucine-rich repeat-containing G-protein-coupled receptor 5 Cancer prognosis Cancer progression 

分 类 号：R735.34[医药卫生—肿瘤]