A Plant Lectin Receptor-like Kinase Phosphorylates the Bacterial Effector AvrPtoB to Dampen Its Virulence in Arabidopsis  

作　　者：Ning Xu Xuming Luo Wei Wu Yingying Xing Yingbo Liang Yanzhi Liu Huasong Zou Hai-Lei Wei Jun Liu 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [2]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China [3]College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China [4]Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture and Rural Affairs,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Molecular Plant》2020年第10期1499-1512,共14页分子植物（英文版）

基　　金：Chinese Academy of Sciences(Strategic Pri-ority Research Program grant no.XDB11020300);the National Key R&D Program of China(2017YFD0200900);the Natural Science Foundation of China(31972257);the State Key Laboratory of Plant Genomics(Grant No.O8KF021011)to J.L.

摘　　要：Plasma membrane-localized receptor-like kinases(RLKs)perceive conserved pathogen-associated molecular pattems(PAMPs)in plants,leading to PAMP-triggered immunity(PTI).TheArabidopsis thaliana lectin RLK LecRK-Ⅸ.2 has been shown to regulate the bacterial flagellin-derived peptide flg22-induced PTI.Here,we discover that Pseudomonas syringae effector AvrPtoB targets LecRK-Ⅸ.2 for degradation,which subsequently suppresses LecRK-Ⅸ.2-mediated PTI and disease resistance.However,LecRK-Ⅸ.2 can interact with and phosphorylate AvrPtoB at serine site 335(S335).AvrPtoB self-associates in vitro and in vivo,and the association appears to be essential for its E3 ligase activity in ubiquitinating substrate in plants.Phosphorylation of S335 disrupts the self-association and as a result,phosphomimetic AvrPtoBS335D cannot ubiquitinate LecRK-Ⅸ.2 efficiently,leading to the compromised virulence of AvrPtoB in suppressing PTI responses.fig22 enhances AvrPtoB S335 phosphorylation by inducing the expression and activating of LecRK-Ⅸ.2.Our study demonstrates that host RLKs can modify pathogen effectors to dampen their virulence and undermine their ability in suppressing PTI.

关 键 词：ARABIDOPSIS AvrPtoB LecRK-Ⅸ.2 PSEUDOMONAS SYRINGAE UBIQUITINATION 

分 类 号：Q94[生物学—植物学]