成对关联刺激对缺血性脑卒中大鼠缺血半暗带区NogoA/NgR/RhoA信号通路蛋白表达的影响  

Effects of paired associative stimulation on the expression of NogoA/NgR/RhoA signaling regulation pathway in ischemic penumbra in rats with focal cerebral ischemia-reperfusion

作　　者：胡艳 郭铁成 张香玉 鲁银山 张秀娟 田峻[2] HU Yan;GUO Tiecheng;ZHANG Xiangyu(Department of Rehabilitation Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030)

机构地区：[1]华中科技大学同济医学院附属同济医院康复医学科,武汉430030 [2]武汉大学中南医院 [3]郑州大学第五附属医院

出　　处：《中国康复医学杂志》2020年第12期1409-1415,共7页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金项目(81272156)。

摘　　要：目的:观察成对关联刺激对缺血性脑卒中大鼠缺血半暗带区NogoA、NgR、RhoA蛋白表达的影响。方法:90只健康雄性SD大鼠随机分为假手术组、模型组和PAS组,每组又分为7d、14d、28d三个亚组(n=10)。模型组和PAS组大鼠采用Longa线栓法制作MCAO模型,假手术组除不插入线栓外,其余操作与模型组和PAS组相同。PAS组于术后第1d开始给予0.05Hz、共90对脉冲的PAS治疗,每日1次,每次持续30min;模型组和假手术组不予任何干预措施,各时间点治疗结束后取缺血半暗带区脑组织,Western Blot和免疫荧光检测缺血半暗带NogoA、NgR、RhoA蛋白的表达和分布情况。结果:术后第7、14、28d,假手术组NogoA、NgR、RhoA蛋白的表达水平均最低,模型组在各时间点NogoA、NgR、RhoA含量均较假手术组明显升高(P<0.05),PAS组术后各时间点NogoA、NgR、RhoA的含量高于假手术组(P<0.05),低于同时间点模型组(P<0.05),PAS组NogoA、NgR、RhoA蛋白在术后28d的含量低于同组第7d、14d(P<0.05)。结论:PAS可抑制NogoA/NgR/RhoA信号通路蛋白的表达,可能是PAS促进脑缺血大鼠神经再生,改善脑缺血大鼠的感觉运动功能障碍的内在分子机制之一。Objective:To study the effect of paired associative stimulation(PAS)on the expression of NogoA,NgR,RhoA in ischemic penumbra in rats with middle cerebral artery occlusion(MCAO).Method:Ninety male adult Sprague-Dawley rats were randomly divided into a sham operation group(Sham group),model group(Model group)and paired associative stimulation group(PAS group).Each group was then subdivided into 7-,14-,and 28-day subgroups with 10 rats in each.The focal cerebral ischemia-reperfusion model was established by Longa suture method in model group and PAS group.The rats in sham operation group underwent the same operation procedure except for the real occlusion of the middle cerebral artery.The rats of PAS group received PAS treatment beginning 24h after the occlusion,sham group and model group weren't given any intervention.Rats in each subgroup were sacrificed in a random order on the 7th,14th and 28th days,the expression of NogoA,NgR,RhoA protein in ischemic penumbra were detected using Western Blot and immunofluorescence.Result:The levels of NogoA,NgR and RhoA were the lowest in the sham group at 7,14 and 28 days after operation.The expression of NogoA,NgR and RhoA in the model group were significantly higher than those in the sham group(P<0.05).The contents of NogoA,NgR and RhoA in the PAS group were significantly higher than those in the sham group(P<0.05),but lower than those in the model group(P<0.05).The expression of NogoA,NgR and RhoA in PAS group at 14,28d were lower than those at 7d.Conclusion:PAS can inhibit the expression of NogoA/NgR/RhoA signaling pathway protein,which may be one of the internal molecular mechanisms of PAS to promote nerve regeneration and improve sensory motor dysfunction in cerebral ischemia rats.

关 键 词：脑缺血 成对关联刺激 NogoA NGR RHOA 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R49[医药卫生—临床医学]