AlCl3 exposure regulates neuronal development by modulating DNA modification  

作　　者：Xue-Jun Cheng Fu-Lai Guan Qian Li Gong Dai Hai-Feng Li Xue-Kun Li 

机构地区：[1]The Children's Hospital,School of Medicine,Zhejiang University,Hangzhou 310052,Zhejiang Province,China [2]The Institute of Translational Medicine,School of Medicine,Zhejiang University,Hangzhou 310029,Zhejiang Province,China [3]National Clinical Research Center for Child Health,Hangzhou 310052,Zhejiang Province,China [4]School of Basic Medicine,Weifang Medical University,Weifang 261053,Shandong Province,China

出　　处：《World Journal of Stem Cells》2020年第11期1354-1365,共12页世界干细胞杂志（英文版）（电子版）

摘　　要：BACKGROUND As the third most abundant element,aluminum is widespread in the environment.Previous studies have shown that aluminum has a neurotoxic effect and its exposure can impair neuronal development and cognitive function.AIM To study the effects of aluminum on epigenetic modification in neural stem cells and neurons.METHODS Neural stem cells were isolated from the forebrain of adult mice.Neurons were isolated from the hippocampi tissues of embryonic day 16-18 mice.AlCl3 at 100 and 200μmol/L was applied to stem cells and neurons.RESULTS Aluminum altered the differentiation of adult neural stem cells and caused apoptosis of newborn neurons while having no significant effects on the proliferation of neural stem cells.Aluminum application also significantly inhibited the dendritic development of hippocampal neurons.Mechanistically,aluminum exposure significantly affected the levels of DNA 5-hydroxy methylcytosine,5-methylcytosine,and N6-methyladenine in stem cells and neurons.CONCLUSION Our findings indicate that aluminum may regulate neuronal development by modulating DNA modifications.

关 键 词：ALUMINUM DNA demethylation 5-hydroxymethylcytosine Neural stem cells NEURON Neuronal development 

分 类 号：R114[医药卫生—卫生毒理学]